Mouse anti-Human NPM1 Monoclonal Antibody | anti-NPM1 antibody

NPM1 Antibody

Cat. Num. AAA9200102

• Gene Names: NPM1; B23; NPM
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: NPM1; Monoclonal Antibody; NPM1 Antibody; Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; anti-NPM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1042CT5.2.1
• Specificity: Purified His-tagged NPM1 protein was used to produced this monoclonal antibody.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 259

• Applicable Applications for anti-NPM1 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC~~1:25; WB~~1:1000
• Antigen Type: Recombinant Protein
• Crown Antibody: Yes
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. thymus section using NPM1 Antibody. MBS9200102 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Western Blot (WB)

(NPM1 Antibody western blot analysis in Hela,Jurkat,MCF-7,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the NPM1 antibody detected the NPM1 protein (arrow).)

Related Product Information for anti-NPM1 antibody

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication.

Product Categories/Family for anti-NPM1 antibody

Crown Antibodies

References

Swaminathan V., et al. Mol. Cell. Biol. 25:7534-7545(2005).
Tago K., et al. Proc. Natl. Acad. Sci. U.S.A. 102:7689-7694(2005).
Bolli N., et al. Lancet Oncol. 7:350-352(2006).
Lu L., et al. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 83641870
• NCBI GeneID: 4869
• NCBI Accession #: NP_001032827.1; NP_002511.1; NP_954654.1
• NCBI GenBank Nucleotide #: NM_001037738.2
• UniProt Accession #: P06748; P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6
• Molecular Weight: 32575
• NCBI Official Full Name: nucleophosmin isoform 3
• NCBI Official Synonym Full Names: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin
• UniProt Protein Name: Nucleophosmin
• Protein Family: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM
• UniProt Entry Name: NPM_HUMAN

NCBI Description

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: Translation; RNA-binding; Oncoprotein; Nucleolus

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: NF-kappaB binding; protein binding; protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; Tat protein binding; protein heterodimerization activity; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal large subunit binding; ribosomal small subunit binding

Biological Process: regulation of centriole replication; positive regulation of translation; viral reproduction; centrosome cycle; cell aging; nucleocytoplasmic transport; signal transduction; DNA repair; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; intracellular protein transport; nucleosome assembly; protein localization; regulation of endodeoxyribonuclease activity; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis