Rat anti-Human Notch2 Monoclonal Antibody | anti-NOTCH2 antibody
Notch2 (Notch Homolog 2, Drosophila, hN2)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Western Blot: 1-2ug/ml; With an appropriate colorimetric detection system, the detection limit for rhNotch-2 ICD is ~5ng/lane under non-reducing and reducing conditions.
ELISA: 0.5-1ug/ml; Detection limit for rhNotch-2 ICD is ~12ng/well.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.
Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1p13-p11
Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; membrane; integral to plasma membrane; plasma membrane; extracellular region; nucleus; receptor complex; cilium
Molecular Function: protein binding; calcium ion binding; receptor activity
Biological Process: positive regulation of apoptosis; apoptosis; multicellular organismal development; myeloid dendritic cell differentiation; cell fate determination; negative regulation of transcription from RNA polymerase II promoter; bone remodeling; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; morphogenesis of an epithelial sheet; hemopoiesis; cell growth; cell cycle arrest; positive regulation of BMP signaling pathway; embryonic limb morphogenesis; transcription initiation from RNA polymerase II promoter; nervous system development; Notch signaling pathway; intracellular receptor-mediated signaling pathway; in utero embryonic development; stem cell maintenance; Notch receptor processing; humoral immune response; organ morphogenesis; inflammatory response to antigenic stimulus; defense response to bacterium; gene expression; positive regulation of Ras protein signal transduction; determination of left/right symmetry; negative regulation of apoptosis
Disease: Hajdu-cheney Syndrome; Alagille Syndrome 2
Research Articles on NOTCH2
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Product Notes
The NOTCH2 notch2 (Catalog #AAA603501) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Notch2 (Notch Homolog 2, Drosophila, hN2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Notch2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot and ELISA. Dilution: Western Blot: 1-2ug/ml; With an appropriate colorimetric detection system, the detection limit for rhNotch-2 ICD is ~5ng/lane under non-reducing and reducing conditions. ELISA: 0.5-1ug/ml; Detection limit for rhNotch-2 ICD is ~12ng/well. Researchers should empirically determine the suitability of the NOTCH2 notch2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Notch2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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