Chicken PKIbeta ELISA Kit | PKIbeta elisa kit

Chicken PKIbeta (Protein Kinase Inhibitor Beta) ELISA Kit

Cat. Num. AAA2502738

• Gene Names: Pkib; Pkibeta; Prkacn2
• Reactivity: Chicken
• Synonyms: PKIbeta; Chicken PKIbeta (Protein Kinase Inhibitor Beta) ELISA Kit; PKIbeta elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken PKIbeta. No significant cross-reactivity or interference between Chicken PKIbeta and analogues was observed.
• Sequence Length: 78

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKIbeta elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken PKIbeta concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PKIbeta. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PKIbeta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PKIbeta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PKIbeta. You can calculate the concentration of PKIbeta in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 568965133
• NCBI GeneID: 18768
• NCBI Accession #: XP_006512668.1
• NCBI GenBank Nucleotide #: XM_006512605.2
• UniProt Accession #: Q04758
• Molecular Weight: 7,529 Da
• NCBI Official Full Name: cAMP-dependent protein kinase inhibitor beta isoform X5
• NCBI Official Synonym Full Names: protein kinase inhibitor beta, cAMP dependent, testis specific
• NCBI Official Symbol: Pkib
• NCBI Official Synonym Symbols: Pkibeta; Prkacn2
• NCBI Protein Information: cAMP-dependent protein kinase inhibitor beta; PKI-beta; cAMP-dependent protein kinase inhibitor, testis isoform; protein kinase, cAMP-dependent, catalytic, inhibitor 2
• UniProt Protein Name: cAMP-dependent protein kinase inhibitor beta
• UniProt Gene Name: Pkib
• UniProt Entry Name: IPKB_MOUSE

Uniprot Description

PKIB: Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains. Belongs to the PKI family.

Protein type: Protein kinase, regulatory subunit; Inhibitor

Cellular Component: cytoplasm; nucleus

Molecular Function: protein kinase inhibitor activity; cAMP-dependent protein kinase inhibitor activity

Biological Process: negative regulation of protein kinase activity