NOS Clone 2H11-D11 Monoclonal Antibody
Anti-human inducible Nitric Oxide Synthase Monoclonal Antibody 2H11-D11
Reactivity
HumanCrossreactivity Note: No cross reaction with nNOS or eNOS
Applications
Immunofluorescent Staining of Induced Cells
Synonyms
NOS Clone 2H11-D11; Monoclonal Antibody; Anti-human inducible Nitric Oxide Synthase Monoclonal Antibody 2H11-D11; anti-NOS Clone 2H11-D11 antibody
Reactivity
Human
Crossreactivity Note: No cross reaction with nNOS or eNOS
Crossreactivity Note: No cross reaction with nNOS or eNOS
Clonality
Monoclonal
Isotype
Mouse IgM kappa
Clone Number
2H11-D11
Specificity
Polypeptide ::: % Cross Reactivity
hiNOS ::: 100
rhiNOS (Type II) ::: 100
hiNOS ::: 100
rhiNOS (Type II) ::: 100
Form/Format
Supplied as Culture Supernatant
Applicable Applications for anti-NOS Clone 2H11-D11 antibody
Immunofluorescent Staining of Induced Cells
Application Notes
Immunofluorescent: This monoclonal antibody has been found to stain cells induced to produce iNOS at a 1:20 dilution. The ability of this monoclonal antibody to bind to iNOS in fixed cells was examined using two different cell lines. A-172 (a human glioblastoma cell line). and RAW 264.7 (a mouse macrophage cell line). The cells were cultured for 2 day in normal medium and then induced to produce iNOS by treatment for 40 hours with a cytokine/LPS mixture. Following the induction. the cells were washed x 4 and fixed in either 70% or 100% acetone. They were reacted for 120 minutes with the culture supernatant. and then with FITC-conjugated goat anti-mouse IgM. The immunofluorescent staining pattern was observed using epifluorescent microscopy. Specificity for hiNOS has been determined by lack of immunocytochemical staining of cells know to produce either Type I or Type III NOS
Related Product Information for anti-NOS Clone 2H11-D11 antibody
This culture supernatant contains mouse monoclonal antibody clone 2H11-D11 raised against recombinant hiNOS. This monoclonal antibody has been found to stain hiNOS by immunocytochemistry. This monoclonal antibody was tested for recognition of other NOS isoforms by ELISA and immunocytochemical techniques. It has been isotyped as an IgM class antibody. This monoclonal antibody has been found to immunoprecipitate 125I-hiNOS by RIA techniques
Product Categories/Family for anti-NOS Clone 2H11-D11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
NOS
Similar Products
Product Notes
The NOS Clone 2H11-D11 (Catalog #AAA350051) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-human inducible Nitric Oxide Synthase Monoclonal Antibody 2H11-D11 reacts with Human Crossreactivity Note: No cross reaction with nNOS or eNOS and may cross-react with other species as described in the data sheet. AAA Biotech's NOS Clone 2H11-D11 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescent Staining of Induced Cells. Immunofluorescent: This monoclonal antibody has been found to stain cells induced to produce iNOS at a 1:20 dilution. The ability of this monoclonal antibody to bind to iNOS in fixed cells was examined using two different cell lines. A-172 (a human glioblastoma cell line). and RAW 264.7 (a mouse macrophage cell line). The cells were cultured for 2 day in normal medium and then induced to produce iNOS by treatment for 40 hours with a cytokine/LPS mixture. Following the induction. the cells were washed x 4 and fixed in either 70% or 100% acetone. They were reacted for 120 minutes with the culture supernatant. and then with FITC-conjugated goat anti-mouse IgM. The immunofluorescent staining pattern was observed using epifluorescent microscopy. Specificity for hiNOS has been determined by lack of immunocytochemical staining of cells know to produce either Type I or Type III NOS. Researchers should empirically determine the suitability of the NOS Clone 2H11-D11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOS Clone 2H11-D11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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