Western Blot (WB)
(Western blot of postnatal day 1 rat brain lysate. The blot was probed with mouse monoclonal anti-Nogo A (Central region) antibody at 1:500.)
Mouse Nogo A Monoclonal Antibody | anti-NogoA antibody
Nogo A (Central region) Antibody
Gene Names
Rtn4; r; Nogo; Vp20; rat N; NI-250; Nogo-A; rat NogoA
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
Nogo A; Monoclonal Antibody; Nogo A (Central region) Antibody; Nogo A (Central region); Nogo; anti-NogoA antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M364
Specificity
This antibody detects a 180kDa* protein corresponding to the molecular mass of Nogo A on SDS-PAGE immunoblots of postnatal day 1 rat brain.
Form/Format
Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-NogoA antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:1000
WB: 1:500
WB: 1:500
Immunogen
Clone M364 was generated from a sequence corresponding to amino acids in the central region of rat Nogo A. This sequence has high homology to human and mouse Nogo A.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of postnatal day 1 rat brain lysate. The blot was probed with mouse monoclonal anti-Nogo A (Central region) antibody at 1:500.)
Western Blot (WB)
(Western blot of postnatal day 1 rat brain lysate. The blot was probed with mouse monoclonal anti-Nogo A (Central region) antibody at 1:500.)
Related Product Information for anti-NogoA antibody
After central nervous system (CNS) injury, there is minimal regeneration of axonal projections through the injured region, which compromises functional recovery from these injuries. Various inhibitory molecules found in the myelin of axons in the CNS may limit regeneration through activation of inhibitory cell signaling pathways. Nogo was identified in myelin extracts using the monoclonal antibody IN-1, an antibody that allows modest axon regeneration after spinal cord injury. Nogo is expressed as three different proteins, Nogo-A, -B, and -C, which are members of the Reticulon family of ER anchoring proteins. Nogo-A is the full length protein, while Nogo-B contains 172 amino acids of the N-terminus and 188 amino acids of the C-terminus of Nogo-A, while Nogo-C contains only the 188 amino acid C-terminus of Nogo-A. These splice variants are all found in optic nerve, spinal cord, and cerebral cortex, but differ in expression in other neuronal and non-neuronal tissues. Thus, Nogo-A may be an important myelin-associated protein limiting axon regeneration after CNS injury.
References
Chen, M.S. et al. (2000) Science 403:434.
GrandPre, T. et al.(2000) Nature 403:439.
Tessier-Lavigne, M. & Goodman, C.S. (2000) Science 287:813.
GrandPre, T. et al.(2000) Nature 403:439.
Tessier-Lavigne, M. & Goodman, C.S. (2000) Science 287:813.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
126,388 Da
NCBI Official Full Name
reticulon-4
NCBI Official Synonym Full Names
reticulon 4
NCBI Official Symbol
Rtn4
NCBI Official Synonym Symbols
r; Nogo; Vp20; rat N; NI-250; Nogo-A; rat NogoA
NCBI Protein Information
reticulon-4; foocen; NI-220/250; nogo protein; GLUT4 vesicle 20kDa protein; neurite outgrowth inhibitor; glut4 vesicle 20 kDa protein
UniProt Protein Name
Reticulon-4
UniProt Gene Name
Rtn4
UniProt Synonym Gene Names
Nogo; Nogo protein
UniProt Entry Name
RTN4_RAT
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Product Notes
The NogoA rtn4 (Catalog #AAA474469) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nogo A (Central region) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nogo A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:1000 WB: 1:500. Researchers should empirically determine the suitability of the NogoA rtn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nogo A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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