Mouse anti-Human NOD2 Monoclonal Antibody | anti-NOD2 antibody

NOD2, Human, mAb hNOD2.017

Cat. Num. AAA584763

• Gene Names: NOD2; CD; ACUG; BLAU; IBD1; YAOS; BLAUS; NLRC2; NOD2B; CARD15; CLR16.3; PSORAS1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: NOD2; Monoclonal Antibody; Human; mAb hNOD2.017; CARD15; Caspase recruitment domain-containing protein 15; Inflammatory bowel disease protein 1; anti-NOD2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: hNOD2.017
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 273

• Applicable Applications for anti-NOD2 antibody: Immunohistochemistry (IHC) Formalin/Paraffin, Immuno Assay (IA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IHC-P: Tissue sections were pretreated with 10mM Citrate (pH 6.0). Tissue sections were fixed in alcohol and blocked with endogenous perioxidases.; The typical starting working dilution is 1:50.
• Immunogen: Recombinant NOD2
• Preparation and Storage: Store at 4 degree C. Under recommended storage conditions, product is stable for at least one year.

Related Product Information for anti-NOD2 antibody

Monoclonal antibody 2.017 recognizes human NOD2, also known as CARD15. The innate immune system is the first line of defense against pathogens using various pattern recognition receptors (PRR). PRRs include toll-like receptors (TLRs), nucleotide-biding oligomerization domain (NOD)-like receptors (NLRs) and retinoic acid-inducible (RIG)-I-like receptors (RLR). The Nod-like receptor (NLR) family of proteins consists of cytosolic pattern recognition molecules involved in sensing microbial and danger signals and triggering innate immune activation. The NOD family is involved in the regulation of apoptosis and the immune response. The founding members of NLRs are NOD1 and NOD2. These intracellular receptors recognize peptidoglycans. The receptor consists of three main domains. The C-terminal site recognizes the ligand via the leucin-rich repeat domain (LRR). The NOD domain facilitates the self-oligomerization and contains the ATPase activity. The N-terminal site is the caspase-recruitment domain (CARD). Proteoglycans are polymers composed of glycan chains of alternating N-acylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) units cross-linked to each other by short peptides. The cross-linking of two parallel glycan chains is mediated by stem peptides that can be further linked by bridging amino acids. For activation of the immune system, NOD2 senses muramyl dipeptide (MDP), which is found in the PGN of nearly all Gram-positive and Gram-negative organisms, whereas NOD1 recognizes meso-diaminopimelic acid (meso-DAP)-containing PGN fragments. Stimulation of NOD1 or NOD2 results in the activation of NF-kappaB and MAPKs, through a homophilic interaction with the adaptor kinase, Rip2. Thereby driving the transcription of numerous genes involved in both innate and adaptive immune responses. NOD2 seems of special importance in commensal homeostasis and especially in the gut. This is reflected by the fact that genetic variation has been found in patients with inflammatory bowel disease (IBD), particularly Crohn's disease. NOD2 is mainly expressed by macrophages, monocytes as well as Paneth cells, T and B cell, dendritic cells and intestinal epithelial cells. NOD2 antibody 2.017 can be used for western blotting, immunohistochemistry, ELISA and immunoprecipitation.

NCBI and Uniprot Product Information

• NCBI GI #: 14595638
• NCBI GeneID: 64127
• Molecular Weight: 22,403 Da
• NCBI Official Full Name: NOD2, partial
• NCBI Official Synonym Full Names: nucleotide binding oligomerization domain containing 2
• NCBI Official Symbol: NOD2
• NCBI Official Synonym Symbols: CD; ACUG; BLAU; IBD1; YAOS; BLAUS; NLRC2; NOD2B; CARD15; CLR16.3; PSORAS1
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 2
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 2
• Protein Family: Nucleotide-binding oligomerization domain-containing protein
• UniProt Gene Name: NOD2
• UniProt Synonym Gene Names: CARD15; IBD1

NCBI Description

This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

NOD2: Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK- gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash. Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1). IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Defects in NOD2 are the cause of sarcoidosis early-onset (EOS). EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early- onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement. 2 isoforms of the human protein are produced by alternative initiation.

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: basolateral plasma membrane; cell surface; cytoplasm; cytoskeleton; cytosol; plasma membrane; protein complex; signalosome; vesicle

Molecular Function: actin binding; CARD domain binding; enzyme binding; Hsp70 protein binding; Hsp90 protein binding; muramyl dipeptide binding; peptidoglycan binding; protein binding; protein kinase binding

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; cytokine production during immune response; cytokine secretion during immune response; defense response; defense response to bacterium; detection of bacterium; detection of biotic stimulus; detection of muramyl dipeptide; innate immune response; JNK cascade; maintenance of gastrointestinal epithelium; positive regulation of B cell activation; positive regulation of dendritic cell antigen processing and presentation; positive regulation of epithelial cell proliferation; positive regulation of gamma-delta T cell activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-10 production; positive regulation of interleukin-17 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of Notch signaling pathway; positive regulation of oxidoreductase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of stress-activated MAPK cascade; positive regulation of T-helper 2 type immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; protein oligomerization; regulation of inflammatory response; response to muramyl dipeptide

Disease: Blau Syndrome; Inflammatory Bowel Disease 1; Psoriatic Arthritis, Susceptibility To; Sarcoidosis, Early-onset