Rabbit anti-Human , Rat BRCA2 Polyclonal Antibody | anti-BRCA2 antibody

BRCA2

Cat. Num. AAA127140

• Gene Names: BRCA2; FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
• Reactivity: Human , Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: BRCA2; Polyclonal Antibody; anti-BRCA2 antibody

• Host: Rabbit
• Reactivity: Human , Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Sequence Length: 3418

• Applicable Applications for anti-BRCA2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IF: 1:50 - 1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BRCA2 (NP_000050.2)
• Category: Primary antibody
• Cross Reactivity: Human, Rat
• Preparation and Storage: Store at -20°C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of 293T cells, using BRCA2 antibody (MBS127140) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of C6 cells using BRCA2 antibody (MBS127140) at dilution of 1:100. Blue: DAPI for nuclear staining.)

Related Product Information for anti-BRCA2 antibody

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.

Product Categories/Family for anti-BRCA2 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 14424438
• NCBI GeneID: 675
• UniProt Accession #: P51587; O00183; O15008; Q13879; Q5TBJ7
• Molecular Weight: Observed MW: 384 kDa; Calculated MW: 384 kDa
• NCBI Official Full Name: Breast cancer type 2 susceptibility protein
• NCBI Official Synonym Full Names: breast cancer 2, early onset
• NCBI Official Symbol: BRCA2
• NCBI Official Synonym Symbols: FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
• NCBI Protein Information: breast cancer type 2 susceptibility protein; Fanconi anemia group D1 protein; breast cancer 2 tumor suppressor; BRCA1/BRCA2-containing complex, subunit 2; breast and ovarian cancer susceptibility gene, early onset
• UniProt Protein Name: Breast cancer type 2 susceptibility protein
• Protein Family: BRCA2 and CDKN1A-interacting protein
• UniProt Gene Name: BRCA2
• UniProt Synonym Gene Names: FACD; FANCD1
• UniProt Entry Name: BRCA2_HUMAN

NCBI Description

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]

Uniprot Description

BRCA2: involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation. Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoint functions. Interacts with WDR16. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer and may underlie susceptibility to uveal melanoma.

Protein type: Tumor suppressor; Nuclear receptor co-regulator; DNA repair, damage

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; centrosome; protein complex; cytoplasm; BRCA2-MAGE-D1 complex; nucleus; secretory granule

Molecular Function: gamma-tubulin binding; protein binding; H4 histone acetyltransferase activity; histone acetyltransferase activity; protease binding; H3 histone acetyltransferase activity; single-stranded DNA binding

Biological Process: positive regulation of transcription, DNA-dependent; cytokinesis; cell aging; positive regulation of mitotic cell cycle; DNA repair; regulation of cytokinesis; oocyte maturation; inner cell mass cell proliferation; negative regulation of mammary gland epithelial cell proliferation; response to UV-C; double-strand break repair via homologous recombination; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; male meiosis I; nucleotide-excision repair; double-strand break repair; response to gamma radiation; hemopoiesis; spermatogenesis; replication fork protection; brain development; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; centrosome duplication; female gonad development; response to X-ray

Disease: Pancreatic Cancer, Susceptibility To, 2; Breast-ovarian Cancer, Familial, Susceptibility To, 2; Prostate Cancer; Breast Cancer; Medulloblastoma; Glioma Susceptibility 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group D1; Wilms Tumor 1