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Testing Data (Staining of bovine peripheral blood lymphocytes withMBS631471. A small positive peak representing NK cells can be identified)

Mouse anti-Bovine NKp46 Monoclonal Antibody | anti-NKp46 antibody

NKp46, Natural Cytotoxicity Receptor (NCR NKp46, CD335, Lymphocyte Antigen 94, Ly94, Natural Cytotoxicity Triggering Receptor 1, NCR1, NCT1, Natural Killer Cell Activating Receptor NKp46, NK Cell Activating Receptor) (Biotin)

Reactivity
Bovine
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
NKp46; Monoclonal Antibody; Natural Cytotoxicity Receptor (NCR NKp46; CD335; Lymphocyte Antigen 94; Ly94; Natural Cytotoxicity Triggering Receptor 1; NCR1; NCT1; Natural Killer Cell Activating Receptor NKp46; NK Cell Activating Receptor) (Biotin); anti-NKp46 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Bovine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10B1431
Specificity
Recognizes bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. Species Crossreactivity: American bison
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Applicable Applications for anti-NKp46 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Immunogen
Fusion protein consisting of the extracellular region of bovine CD335.
Conjugate
Biotin
Preparation and Storage
Store at -20 degree C.

Testing Data

(Staining of bovine peripheral blood lymphocytes withMBS631471. A small positive peak representing NK cells can be identified)

Testing Data (Staining of bovine peripheral blood lymphocytes withMBS631471. A small positive peak representing NK cells can be identified)
Product Categories/Family for anti-NKp46 antibody
References
1. Storset, A.K. et al. (2004) NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics. Eur J Immunol. 34 (3): 669-76. 2. Kulberg, S. et al. (2004) Reference values for relative numbers of natural killer cells in cattle blood. Dev Comp Immunol. 28 (9): 941-8. 3. Van Rhijn, I. et al. (2007) Massive, sustained gamma/delta T cell migration from the bovine skin in vivo. J Leukoc Biol. 81: 968-73. 4. Toka, F.N. et al. (2011) Rapid and Transient Activation of gamma/delta T Cells to IFN-gamma Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection. J Immunol. 186: 4853-61. 5. Bastos, R.G. et al. (2008) Bovine NK cells acquire cytotoxic activity and produce IFN-gamma after stimulation by Mycobacterium bovis BCG- or Babesia bovis-exposed splenic dendritic cells. Vet Immunol Immunopathol. 124: 302-12. 6. Boysen, P. et al. (2006) Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status. BMC Immunol. 7:10. 7. Elhmouzi-Younes, J. et al. (2009) Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation. Vet Res. 40: 54. 8. Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells. Vet Res. 41:4. 9. Hoek, A. et al. (2009) Subpopulations of bovine WC1(+) gamma/delta T cells rather than CD4(+)CD25(high) Foxp3(+) T cells act as immune regulatory cells ex vivo. Vet Res. 40:6. 10. Zuerner, R.L. et al. (2011) A Leptospira borgpetersenii Serovar Hardjo Vaccine Induces a Th1 Response, Activates NK Cells, and Reduces Renal Colonization. Clin Vaccine Immunol. 18: 684-91. 11. Nelson, D.D. et al. (2010) CD8(+)/perforin(+)/WC1(-) gamma/delta T cells, not CD8(+) alpha/beta T cells, infiltrate vasculitis lesions of American bison (Bison bison) with experimental sheep-associated malignant catarrhal fever. Vet Immunol Immunopathol. 136: 284-91. 12. Banos, G. et al. (2013) Identification of immune traits correlated with dairy cow health, reproduction and productivity. PLoS One. 8: e65766. 13. Ramstead, A.G. et al. (2015) Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B. Int Immunopharmacol. 26 (2): 367-77. 14. Boysen, P. et al. (2008) Natural killer cells in lymph nodes of healthy calves express CD16 and show both cytotoxic and cytokine-producing properties. Dev Comp Immunol. 32 (7): 773-83. 15. Gonzalez-Cano, P. et al. (2014) Two functionally distinct myeloid dendritic cell subpopulations are present in bovine blood. Dev Comp Immunol. 44 (2): 378-88. 16. Di Palma, S. et al. (2012) Comparative spatiotemporal analysis of the intrathecal immune response in natural listeric rhombencephalitis of cattle and small ruminants. Comp Immunol Microbiol Infect Dis. 35 (5): 429-41. 17. Pirson, C. et al. (2015) Highly purified mycobacterial phosphatidylinositol mannosides drive cell-mediated responses and activate NKT cells in cattle. Clin Vaccine Immunol. 22 (2): 178-84. 18. Schneider, D.A. et al. (2011) Dynamics of bovine spleen cell populations during the acute response to Babesia bovis infection: an immunohistological study. Parasite Immunol. 33 (1): 34-44. 19. Van Meulder F et al. (2015) Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasite Cooperia oncophora. Int J Parasitol. 45 (9-10): 637-46. 20. Patch, J.R. et al. (2014) Infection with foot-and-mouth disease virus (FMDV) induces a natural killer (NK) cell response in cattle that is lacking following vaccination. Comp Immunol Microbiol Infect Dis. 37 (4): 249-57. 21. Hedges, J.F. et al. (2015) Amphotericin B stimulates gamma delta T and NK cells, and enhances protection from Salmonella infection. Innate Immun. pii: 1753425914567692. 22. Allan AJ et al. (2015) Cattle NK Cell Heterogeneity and the Influence of MHC Class I. J Immunol. pii: 1500227. 1. Sivori, S. et al. (1997) p46, a novel natural killer cell-specific surface molecule that mediates cell activation. J Exp Med. 186 (7): 1129-36. 2. Storset, A.K. et al. (2003) Natural killer cell receptors in cattle: a bovine killer cell immunoglobulin-like receptor multigene family contains members with divergent signaling motifs. Eur J Immunol. 33 (4): 980-90.

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Product Notes

The NKp46 (Catalog #AAA6249203) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NKp46, Natural Cytotoxicity Receptor (NCR NKp46, CD335, Lymphocyte Antigen 94, Ly94, Natural Cytotoxicity Triggering Receptor 1, NCR1, NCT1, Natural Killer Cell Activating Receptor NKp46, NK Cell Activating Receptor) (Biotin) reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's NKp46 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the NKp46 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKp46, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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