Rabbit anti-Human SCP2 Polyclonal Antibody | anti-SCP2 antibody

SCP2, NT (SCP2, Non-specific lipid-transfer protein, Propanoyl-CoA C-acyltransferase, SCP-chi, SCPX, Sterol carrier protein 2, Sterol carrier protein X)

Cat. Num. AAA645070

• Gene Names: SCP2; NLTP; SCPX; SCP-2; SCP-X; NSL-TP; SCP-CHI
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SCP2; Polyclonal Antibody; NT (SCP2; Non-specific lipid-transfer protein; Propanoyl-CoA C-acyltransferase; SCP-chi; SCPX; Sterol carrier protein 2; Sterol carrier protein X); Anti -SCP2; anti-SCP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-SCP2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: SCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-43 amino acids from the N-terminal region of human SCP2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SCP2 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SCP2 antibody detected the SCP2 protein (arrow).)

Immunohistochemistry (IHC)

(SCP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SCP2 antibody

This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.

Product Categories/Family for anti-SCP2 antibody

Antibodies; Abs to Enzymes; Acyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 263551
• NCBI GeneID: 6342
• UniProt Accession #: P22307; Q15432; Q16622; Q5VVZ1; Q6NXF4; A6NM69; B4DGJ9; B4DHP6; C9JC79; D3DQ37; E1B6W5; F2Z3J1
• Molecular Weight: 58,994 Da
• NCBI Official Full Name: sterol carrier protein 2
• NCBI Official Synonym Full Names: sterol carrier protein 2
• NCBI Official Symbol: SCP2
• NCBI Official Synonym Symbols: NLTP; SCPX; SCP-2; SCP-X; NSL-TP; SCP-CHI
• NCBI Protein Information: non-specific lipid-transfer protein; sterol carrier protein X; propanoyl-CoA C-acyltransferase
• UniProt Protein Name: Non-specific lipid-transfer protein
• Protein Family: SCP2 sterol-binding domain-containing protein
• UniProt Gene Name: SCP2
• UniProt Synonym Gene Names: NSL-TP; SCP-2; SCP-X
• UniProt Entry Name: NLTP_HUMAN

NCBI Description

This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.[provided by RefSeq, Aug 2010]

Uniprot Description

SCP2: Mediates in vitro the transfer of all common phospholipids, cholesterol and gangliosides between membranes. May play a role in regulating steroidogenesis. Defects in SCP2 are a cause of leukoencephalopathy with dystonia and motor neuropathy (LDMN); also known as sterol carrier protein 2 deficiency. LDMN is a syndrome characterized by leukoencephalopathy, dystonic head tremor, spasmodic torticollis and reduced tendon reflexes in lower extremities. Additional features include hyposmia, pathologic saccadic eye movements, a slight hypoacusis, accumulation of branched-chain pristanic acid in plasma, and the presence of abnormal bile alcohol glucuronides in urine. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Transferase; Lipid Metabolism - primary bile acid biosynthesis; EC 2.3.1.176; Lipid-binding; Mitochondrial

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; peroxisomal matrix; protein complex; mitochondrion; membrane; intracellular membrane-bound organelle; cytoplasm; peroxisome

Molecular Function: protein binding; acyl-CoA binding; cholesterol binding; phosphatidylinositol transporter activity; propanoyl-CoA C-acyltransferase activity; receptor binding

Biological Process: bile acid biosynthetic process; progesterone biosynthetic process; fatty acid beta-oxidation using acyl-CoA oxidase; peroxisome organization and biogenesis; inositol trisphosphate biosynthetic process; bile acid metabolic process; unsaturated fatty acid metabolic process; positive regulation of intracellular cholesterol transport; phospholipid transport; cellular lipid metabolic process; lipid transport; steroid biosynthetic process; positive regulation of steroid metabolic process

Disease: Leukoencephalopathy With Dystonia And Motor Neuropathy