Mouse Nitrotyrosine Monoclonal Antibody
Anti-Nitrotyrosine Monoclonal Antibody
Applications
Immunoblot, Western Blot, Immunohistochemistry, Immunoprecipitation
Synonyms
Nitrotyrosine; Monoclonal Antibody; Anti-Nitrotyrosine Monoclonal Antibody; anti-Nitrotyrosine antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Specificity
Nitrotyrosine [Mouse monoclonal antibody recognizes 3-nitrotyrosine moieties in human, mouse, rat, and dog samples.]
This antibody recognizes 3-nitrotyrosine moieties in human, mouse, rat, and dog samples.
This antibody recognizes 3-nitrotyrosine moieties in human, mouse, rat, and dog samples.
Form/Format
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Applicable Applications for anti-Nitrotyrosine antibody
Immunoblot, Immunohistochemistry, Immunoprecipitation
Application Notes
Immunoblotting: use at 0.2-2ug/ml
Immunohistochemistry: use at 1-5ug/ml.
Immunoprecipitation: use 1-2ug per 100-500ug total protein. These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain tissue extract or nitrated BSA.
Immunohistochemistry: use at 1-5ug/ml.
Immunoprecipitation: use 1-2ug per 100-500ug total protein. These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain tissue extract or nitrated BSA.
Antigen
3-(4-hydroxy-3-nitrophenyl-acetamido) propionic acid conjugated to BSA.
Preservatives
0.09% sodium azide
Other Additives
50% glycerol
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Related Product Information for anti-Nitrotyrosine antibody
Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.
Product Categories/Family for anti-Nitrotyrosine antibody
References
Girault, I et al. 2001 Free Radic Biol Med 31: 1375-87.
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Product Notes
The Nitrotyrosine (Catalog #AAA190493) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nitrotyrosine can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunohistochemistry, Immunoprecipitation. Immunoblotting: use at 0.2-2ug/ml Immunohistochemistry: use at 1-5ug/ml. Immunoprecipitation: use 1-2ug per 100-500ug total protein. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain tissue extract or nitrated BSA. Researchers should empirically determine the suitability of the Nitrotyrosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nitrotyrosine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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