Immunohistochemistry (IHC)
(IHC: Viral infected human heart stained with MBS604292 (1ug/ml))
Mouse Nitrotyrosine Monoclonal Antibody | anti-nTyr antibody
Nitrotyrosine (nTyr) (AP)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Nitrotyrosine; Monoclonal Antibody; Nitrotyrosine (nTyr) (AP); anti-nTyr antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
0.T.98
Specificity
Recognizes nitrated proteins from all vertebrates.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-nTyr antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ICC: 5-10ug/ml showed positive immunostaining for nitrated proteins in peroxynitrite-treated (5-10mM for 5 min) A431 cells fixed with 95% ethanol/5% acetic acid.
WB: Nitrated-BSA
WB: 0.5-2ug/ml detects nitrated proteins in a lysate prepared from peroxynitrite A431 cells. A previous lot detected nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins.
Applications are based on unconjugated antibody.
WB: Nitrated-BSA
WB: 0.5-2ug/ml detects nitrated proteins in a lysate prepared from peroxynitrite A431 cells. A previous lot detected nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins.
Applications are based on unconjugated antibody.
Immunogen
Nitrated Keyhole Limpet Hemocyanin (KLH)
Conjugate
AP
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(IHC: Viral infected human heart stained with MBS604292 (1ug/ml))
Immunohistochemistry (IHC)
(IHC: Viral infected human heart stained with MBS604292 (1ug/ml))
Western Blot (WB)
(Western Blot Analysis: Representative lot data. A431 cell lysate was treated without (lane 1) or with (lane 2) Peroxynitrite. Nitrated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS604292 (0.5ug/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Western Blot (WB)
(Western Blot Analysis: Representative lot data. A431 cell lysate was treated without (lane 1) or with (lane 2) Peroxynitrite. Nitrated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS604292 (0.5ug/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Related Product Information for anti-nTyr antibody
Indirect A549 cells metabolically labeled with 3-nitrosine
Product Categories/Family for anti-nTyr antibody
References
1. MacMillan-Crow, L.A., et al., Proc. Natl. Acad. Sci. USA 93: 11853-11858, 1996. 2. Beckman, J.S., et al., Biol. Chem. Hoppe-Seyler 375: 81-88, 1994. 3. Beckman, J.S., et al., Nature 364: 584, 1993. 4. Ischiropoulos, H., et al., Arch. Biochem, Biophys. 298: 431-437, 1992. 5. Ohshima, H., et al., Ed. Chem. Tox. 28: 647-652, 1990.
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Product Notes
The nTyr (Catalog #AAA6241511) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nitrotyrosine can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). ICC: 5-10ug/ml showed positive immunostaining for nitrated proteins in peroxynitrite-treated (5-10mM for 5 min) A431 cells fixed with 95% ethanol/5% acetic acid. WB: Nitrated-BSA WB: 0.5-2ug/ml detects nitrated proteins in a lysate prepared from peroxynitrite A431 cells. A previous lot detected nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the nTyr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nitrotyrosine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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