Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NIT2 protein (Right) or empty vector (Left) detected with NIT2 antibody)
Mouse NIT2 Monoclonal Antibody | anti-NIT2 antibody
NIT2 antibody
Gene Names
NIT2; HEL-S-8a
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
NIT2 antibody was purified by affinity chromatography.
Synonyms
NIT2; Monoclonal Antibody; NIT2 antibody; Monoclonal NIT2; Anti-NIT2; Nitrilase Family Member 2; MGC111199; NIT 2; NIT-2; anti-NIT2 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3F3
Purity/Purification
NIT2 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
276
Applicable Applications for anti-NIT2 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
IHC: 1:50
WB: 1:2000
IHC: 1:50
WB: 1:2000
Biological Significance
NIT2 belongs to the UPF0012 family and contains 1 CN hydrolase domain. Overexpression of NIT2 decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
Cross-Reactivity
Human
Immunogen
NIT2 antibody was raised in mouse using a full length recombinant protein of human NIT2 (NP_064587) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NIT2 protein (Right) or empty vector (Left) detected with NIT2 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NIT2 protein (Right) or empty vector (Left) detected with NIT2 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NIT2 protein in paraffin embedded Human kidney tissue using NIT2 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NIT2 protein in paraffin embedded Human kidney tissue using NIT2 antibody)
Related Product Information for anti-NIT2 antibody
Mouse monoclonal NIT2 antibody
Product Categories/Family for anti-NIT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,608 Da
NCBI Official Full Name
omega-amidase NIT2
NCBI Official Synonym Full Names
nitrilase family, member 2
NCBI Official Symbol
NIT2
NCBI Official Synonym Symbols
HEL-S-8a
NCBI Protein Information
omega-amidase NIT2
UniProt Protein Name
Omega-amidase NIT2
Protein Family
UniProt Gene Name
NIT2
UniProt Entry Name
NIT2_HUMAN
Uniprot Description
NIT2: a protein of unknown function. A CN hydrolase domain suggests that this protein may be involved in nitrilase-related metabolism and handling.
Protein type: Hydrolase; EC 3.5.1.3; Mitochondrial
Chromosomal Location of Human Ortholog: 3q12.2
Cellular Component: centrosome; mitochondrion; cytoplasm
Molecular Function: omega-amidase activity
Biological Process: oxaloacetate metabolic process; glutamine metabolic process; asparagine metabolic process
Research Articles on NIT2
Similar Products
Product Notes
The NIT2 nit2 (Catalog #AAA833685) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NIT2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 IHC: 1:50 WB: 1:2000. Researchers should empirically determine the suitability of the NIT2 nit2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIT2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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