Rat anti-Mouse, Human Nidogen/Enactin Monoclonal Antibody
Rat Anti-Nidogen/Enactin [JF3]
Reactivity
Mouse, Human
Applications
Western Blot, ELISA
Purity
Protein G
Synonyms
Nidogen/Enactin; Monoclonal Antibody; Rat Anti-Nidogen/Enactin [JF3]; Ent antibody; Entactin 1 antibody; NID 1 antibody; NID antibody; Nid1 antibody; Nidogen (enactin)antibody; Nidogen 1 antibody; Nidogen antibody; anti-Nidogen/Enactin antibody
Host
Rat
Reactivity
Mouse, Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
JF3
Specificity
Recognises native and denatured nidogen 1, and blocks binding of nidogen to laminin. Epitope Located in the G1/Link region of Nidogen 1
Purity/Purification
Protein G
Form/Format
Affinity purified antibody from culture supernatant in 0.5 M acetic acid neutralized with 2 M Trizma
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Nidogen/Enactin antibody
Western Blot (WB), ELISA (EIA), Blocking
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try; BL: Use at an assay dependent dilution. Blocks binding of nidogen to laminin. WB: Use at an assay dependent dilution. Predicted molecular weight: 136 kDa
Target Antigen
Nidogen/Enactin
Immunogen
Recombinant fragment corresponding to the G1/Link region of mouse nidogen
Myeloma/fusion Partners
Fisher rat plus YB2/0
Research Areas
Cell Biology; Cytoskeleton / ECM; Neuroscience
References
Mapping of nidogen binding sites for collagen type IV, heparan sulfate proteoglycan, and zinc. Reinhardt, D. et al. (J Biol Chem,1993) PUBMED
Similar Products
Product Notes
The Nidogen/Enactin (Catalog #AAA375088) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Nidogen/Enactin [JF3] reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nidogen/Enactin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Blocking. Optimal antibody dilution should be determined by titration, however as a guideline try; BL: Use at an assay dependent dilution. Blocks binding of nidogen to laminin. WB: Use at an assay dependent dilution. Predicted molecular weight: 136 kDa. Researchers should empirically determine the suitability of the Nidogen/Enactin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nidogen/Enactin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
