Mouse anti-Human NHERF1 Monoclonal Antibody | anti-NHERF1 antibody

Anti-NHERF1 Purified

Cat. Num. AAA1800570

• Gene Names: SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot, Immunohistochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: NHERF1; Monoclonal Antibody; Anti-NHERF1 Purified; SLC9A3 regulator 1; EBP50; NPHLOP2; SLC9A3R1; SR1; anti-NHERF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: EBP-10
• Specificity: The mouse monoclonal antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 358

• Applicable Applications for anti-NHERF1 antibody: Immunoprecipitation, Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5 ug/ml.; Western blotting: Recommended dilution: 2 ug/ml; 60 min on vertical incubator, positive control: RAJI human lymphoma cell lysate, non-reducing conditions, 10% separating SDS-PAGE gel.
• Immunogen: Bacterially produced recombinant full-length human NHERF1.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Related Product Information for anti-NHERF1 antibody

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

NCBI and Uniprot Product Information

• NCBI GI #: 4759140
• NCBI GeneID: 9368
• NCBI Accession #: NP_004243.1
• NCBI GenBank Nucleotide #: NP_004243.1
• UniProt Accession #: O14745
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• NCBI Official Synonym Full Names: SLC9A3 regulator 1
• NCBI Official Symbol: SLC9A3R1
• NCBI Official Synonym Symbols: EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• UniProt Gene Name: SLC9A3R1
• UniProt Synonym Gene Names: NHERF; NHERF1; NHERF-1; EBP50
• UniProt Entry Name: NHRF1_HUMAN

NCBI Description

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]