Western Blot (WB)
(Western Blot Analysis: Human kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS604289 (1:500). Arrow indicates protein Na+/H+ Exchanger-1 (~100-110kD).)
Mouse NHE1 Monoclonal Antibody | anti-Nhe1 antibody
NHE1 (Na, H Exchanger Isoform)
Gene Names
Nhe1; CG12178; DmelCG12178; DmNHE1; nhe1; NHE1
Reactivity
Avian, Mammal, Porcine
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
NHE1; Monoclonal Antibody; NHE1 (Na; H Exchanger Isoform); Anti -NHE1 (Na; anti-Nhe1 antibody
Host
Mouse
Reactivity
Avian, Mammal, Porcine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q2265
Specificity
Recognizes Na+/H+ exchanger, isoform NHE1 at ~100-110kD. Species Crossreactivity: All vertebrates including fish, amphibian, avian and mammalian.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Applicable Applications for anti-Nhe1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot. Works poorly in Immunohistochemistry and Immunocytochemistry. Not suitable for use in Immunoprecipitation.
Dilution: Western Blot: 1:500 detected NHE1 on 10ug of human kidney lysates. Human kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with N2015-02 (1:500). Proteins were visualized using an IgG (HRP) Gt xMo* and a chemiluminescence detection system.
Dilution: Western Blot: 1:500 detected NHE1 on 10ug of human kidney lysates. Human kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with N2015-02 (1:500). Proteins were visualized using an IgG (HRP) Gt xMo* and a chemiluminescence detection system.
Immunogen
MBP fusion protein containing the entire C-terminal, hydrophilic domain of porcine NHE1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot Analysis: Human kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS604289 (1:500). Arrow indicates protein Na+/H+ Exchanger-1 (~100-110kD).)
Western Blot (WB)
(Western Blot Analysis: Human kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS604289 (1:500). Arrow indicates protein Na+/H+ Exchanger-1 (~100-110kD).)
Related Product Information for anti-Nhe1 antibody
Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue- specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.
Product Categories/Family for anti-Nhe1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Na[+]/H[+] hydrogen exchanger 1
NCBI Official Synonym Full Names
Na[+]/H[+] hydrogen exchanger 1
NCBI Official Symbol
Nhe1
NCBI Official Synonym Symbols
CG12178; DmelCG12178; DmNHE1; nhe1; NHE1
NCBI Protein Information
CG12178 gene product from transcript CG12178-RB; Na[+]/H[+] hydrogen exchanger 1; CG12178-PB; Nhe1-PB
Similar Products
Product Notes
The Nhe1 (Catalog #AAA604289) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NHE1 (Na, H Exchanger Isoform) reacts with Avian, Mammal, Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's NHE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Works poorly in Immunohistochemistry and Immunocytochemistry. Not suitable for use in Immunoprecipitation. Dilution: Western Blot: 1:500 detected NHE1 on 10ug of human kidney lysates. Human kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with N2015-02 (1:500). Proteins were visualized using an IgG (HRP) Gt xMo* and a chemiluminescence detection system. Researchers should empirically determine the suitability of the Nhe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NHE1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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