Mouse NFKBIB Monoclonal Antibody | anti-NFKBIB antibody

NFKBIB (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, beta, IKBB, TRIP9) (PE)

Cat. Num. AAA6186741

• Gene Names: NFKBIB; IKBB; TRIP9
• Applications: Immunofluorescence, Western Blot
• Purity: Purified
• Synonyms: NFKBIB; Monoclonal Antibody; NFKBIB (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor; beta; IKBB; TRIP9) (PE); Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor; TRIP9; anti-NFKBIB antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1G6
• Specificity: Recognizes NFKBIB.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 356

• Applicable Applications for anti-NFKBIB antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: NFKBIB (AAH15528, 1aa-356aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged NFKBIB is 0.1 ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of NFKBIB expression in transfected 293T cell line by NFKBIB monoclonal antibody (M06), clone 1G6.Lane 1: NFKBIB transfected lysate (37.8 KDa).Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to NFKBIB on HeLa cell. [antibody concentration 40 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to NFKBIB on HeLa cell. [antibody concentration 40 ug/ml])

Related Product Information for anti-NFKBIB antibody

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). [supplied by OMIM]

Product Categories/Family for anti-NFKBIB antibody

Antibodies; Protein Kinase Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 15930192
• NCBI GeneID: 4793
• NCBI Official Full Name: Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
• NCBI Official Synonym Full Names: NFKB inhibitor beta
• NCBI Official Symbol: NFKBIB
• NCBI Official Synonym Symbols: IKBB; TRIP9
• NCBI Protein Information: NF-kappa-B inhibitor beta
• Protein Family: NF-kappa-B inhibitor

NCBI Description

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]