Western Blot (WB)
(Western Blot analysis of HeLa nuclear extract using MBS608702 at 0.5ug/ml.)
Mouse anti-Human NFkB Monoclonal Antibody | anti-NFkB antibody
NFkB, p52 (NF Kappa B, NF-kB, Nuclear Factor kappa B) (AP)
WB: 0.5-2ug/ml. Detects NFkB p52 and its p100 precursor in HeLa nuclear extract and human Raji cell but not in mouse 3T3 cell and rat PC 12 cell lysates.
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blot analysis of HeLa nuclear extract using MBS608702 at 0.5ug/ml.)
Western Blot (WB)
(Western Blot analysis of HeLa nuclear extract using MBS608702 at 0.5ug/ml.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the subunits of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB transcription factor complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The NFkB complex can consist of different subunits that form both homo- or heterodimers which bind specific kappa-B elements in target genes. This gene encodes the p100 subunit that is processed into the active p52 subunit. This protein can function as both a transcriptional activator and repressor, depending on its dimer partner. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, May 2012]
Uniprot Description
NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.
Protein type: Transcription factor; Oncoprotein; DNA-binding
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; cytosol; nucleus
Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription factor activity
Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; follicular dendritic cell differentiation; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; response to cytokine stimulus; toll-like receptor signaling pathway; germinal center formation; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway
Disease: Immunodeficiency, Common Variable, 10
Research Articles on NFkB
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Product Notes
The NFkB nfkb2 (Catalog #AAA6241524) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NFkB, p52 (NF Kappa B, NF-kB, Nuclear Factor kappa B) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFkB can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB), Gel Shift Assay. IP: 4ug immunoprecipitates NF-kB p52 and its p100 precursor from 500ug HeLa nuclear extract cell lysate. WB: 0.5-2ug/ml. Detects NFkB p52 and its p100 precursor in HeLa nuclear extract and human Raji cell but not in mouse 3T3 cell and rat PC 12 cell lysates. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NFkB nfkb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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