Mouse NFE2L2 Monoclonal Antibody | anti-NFE2L2 antibody

NFE2L2 (Nuclear Factor (erythroid-Derived 2)-like 2, NRF2) (FITC)

Cat. Num. AAA6175852

• Gene Names: NFE2L2; NRF2
• Applications: ELISA, Western Blot
• Purity: Purified
• Synonyms: NFE2L2; Monoclonal Antibody; NFE2L2 (Nuclear Factor (erythroid-Derived 2)-like 2; NRF2) (FITC); Nuclear Factor (erythroid-Derived 2)-like 2; NRF2; anti-NFE2L2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3G7
• Specificity: Recognizes NFE2L2.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-NFE2L2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: NFE2L2 (NP_006155.2, 351aa-450aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: SPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRL
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NFE2L2 expression in transfected 293T cell line by NFE2L2 monoclonal antibody (M04), clone 3G7.Lane 1: NFE2L2 transfected lysate (67.8 KDa).Lane 2: Non-transfected lysate.)

Related Product Information for anti-NFE2L2 antibody

NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]). [supplied by OMIM]

Product Categories/Family for anti-NFE2L2 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 20149576
• NCBI GeneID: 4780
• NCBI Accession #: NP_006155.2
• NCBI GenBank Nucleotide #: NM_006164.4
• UniProt Accession #: Q16236; Q53RW6; Q59HH2; Q96F71; B2RBU2; B4E338; E9PGJ7
• Molecular Weight: 67,827 Da
• NCBI Official Full Name: nuclear factor erythroid 2-related factor 2 isoform 1
• NCBI Official Synonym Full Names: nuclear factor (erythroid-derived 2)-like 2
• NCBI Official Symbol: NFE2L2
• NCBI Official Synonym Symbols: NRF2
• NCBI Protein Information: nuclear factor erythroid 2-related factor 2; HEBP1; NFE2-related factor 2; NF-E2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid derived 2, like 2
• UniProt Protein Name: Nuclear factor erythroid 2-related factor 2
• Protein Family: Nuclear factor erythroid 2-related factor
• UniProt Gene Name: NFE2L2
• UniProt Synonym Gene Names: NRF2; NF-E2-related factor 2
• UniProt Entry Name: NF2L2_HUMAN

NCBI Description

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; nucleus; chromatin; cytosol

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; positive regulation of blood coagulation; unfolded protein response; regulation of embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter