Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK11 protein using NEK11 antibody)
Mouse NEK11 Monoclonal Antibody | anti-NEK11 antibody
NEK11 antibody
Applications
Immunofluorescence, Western Blot
Purity
NEK11 antibody was purified by affinity chromatography.
Synonyms
NEK11; Monoclonal Antibody; NEK11 antibody; Monoclonal NEK11; Anti-NEK11; Nima; Never In Mitosis Gene A-Related Kinase 11; FLJ23495; NEK 11; NEK-11; anti-NEK11 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4H5
Purity/Purification
NEK11 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
482
Applicable Applications for anti-NEK11 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100
WB: 1:2000
WB: 1:2000
Biological Significance
NEK11 is a member of the never in mitosis gene A family of kinases. NEK11 localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. It appears to play roles in DNA replication and response to genotoxic stress. NEK11 belongs to the NIMA family of kinases, which are involved in DNA replication and genotoxic stress responses.
Cross-Reactivity
Human
Immunogen
NEK11 antibody was raised in mouse using a full length recombinant protein of human NEK11 (NP_079076) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK11 protein using NEK11 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK11 protein using NEK11 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NEK11 protein (Right) or empty vector (Left) detected with NEK11 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NEK11 protein (Right) or empty vector (Left) detected with NEK11 antibody)
Related Product Information for anti-NEK11 antibody
Mouse monoclonal NEK11 antibody
Product Categories/Family for anti-NEK11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
NEK11, partial
Protein Family
Similar Products
Product Notes
The NEK11 (Catalog #AAA837914) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NEK11 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the NEK11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
