Western Blot (WB)
(Figure 1: Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa))
Mouse anti-Human NEFL Monoclonal Antibody | anti-NEFL antibody
NEFL Antibody
Gene Names
NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Affinity-Chromatography
Synonyms
NEFL; Monoclonal Antibody; NEFL Antibody; 150kDa medium; 160 kDa neurofilament protein; 2 kDa neurofilament protein; 200 kDa neurofilament protein; 68 kDa neurofilament protein; AI847934; CMT1F; CMT2CC; CMT2E; FLJ53642; KIAA0845; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; mKIAA0845; Nef3; NEFH; NEFM; Neurofilament 3; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament medium polypeptide 150kDa; Neurofilament medium polypeptide; Neurofilament protein heavy polypeptide; Neurofilament protein light chain; Neurofilament protein light polypeptide; Neurofilament protein medium polypeptide; neurofilament subunit NF-L; Neurofilament triplet H protein; Neurofilament triplet L protein; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); NF-H; NF-L; NF-M; NF160; NF165; NF200; NF68; NFH; NFL; NFM; NFM_HUMAN; PPP1R110; protein phosphatase 1; regulatory subunit 110; anti-NEFL antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
NEFL antibody detects endogenous levels of total NEFL
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
543
Applicable Applications for anti-NEFL antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF/ICC: 1:200-1:1000
FC/FACS: 1:200-1:400
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF/ICC: 1:200-1:1000
FC/FACS: 1:200-1:400
Immunogen
Purified recombinant fragment of human NEFL expressed in E. Coli
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa))
Related Product Information for anti-NEFL antibody
Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Subunit Structure: Interacts with ARHGEF28. Interacts with TRIM2.
Post-translational Modifications: O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Similarity: The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. Belongs to the intermediate filament family.
Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Subunit Structure: Interacts with ARHGEF28. Interacts with TRIM2.
Post-translational Modifications: O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Similarity: The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 62 kDa
Predicted: 62 kDa
Predicted: 62 kDa
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament light
NCBI Official Symbol
NEFL
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110
NCBI Protein Information
neurofilament light polypeptide
UniProt Protein Name
Neurofilament light polypeptide
Protein Family
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF68; NFL; NF-L
NCBI Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Uniprot Description
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Research Articles on NEFL
Similar Products
Product Notes
The NEFL nefl (Catalog #AAA9602388) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NEFL Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEFL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 IF/ICC: 1:200-1:1000 FC/FACS: 1:200-1:400. Researchers should empirically determine the suitability of the NEFL nefl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEFL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
