Rat Nectin-3 Monoclonal Antibody | anti-PVRL3 antibody

Nectin-3, Mouse, mAb 103-A1, FITC

Cat. Num. AAA584065

• Gene Names: PVRL3; PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• Reactivity: Human nectin-3: No; Rat nectin-3: No
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry
• Purity: Protein G
• Synonyms: Nectin-3; Monoclonal Antibody; Mouse; mAb 103-A1; FITC; Poliovirus receptor-related protein 3; Nectin cell adhesion molecule 3; CD113; anti-PVRL3 antibody

• Host: Rat
• Reactivity: Human nectin-3: No; Rat nectin-3: No
• Clonality: Monoclonal
• Isotype: IgG2a
• Purity/Purification: Protein G
• Form/Format: 0.2 um filtered in PBS + 1% BSA + 0.02% NaN3
• Concentration: 100ug/ml (varies by lot)
• Sequence Length: 549

• Applicable Applications for anti-PVRL3 antibody: Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF), Flow Cytometry (FC), Functional Studies (FS), Immuno Assay (IA)
• Application Notes: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. ; ; IF: Fixed in 2% paraformaldehyde in PBS optionally followed by methanol/aceton fixation .; FC: 1 ug per 1 x10 ₆ cells. ; FS: Injection of 1 ug into lumen of seminiferous tubules; IA: Indirect ELISA on 0.5 ug/mL mouse nectin-3 coated per well. Positive signal with 2 ug/mL antibody. ; IF: Cells are fixed in 2% paraformaldehyde in PBS, optionally followed by methanol/aceton fixation. Proteins were seperated by SDS-PAGE and electrophoretically transfered onto polyvinylidene floride sheets, which then incubate with primary antibodies. Binding was detected with HRP-conjugated secondary antibodies and Western blotting detection reagents; PC: L cells (A cultured line of C3H mouse fibroblasts); Negative control: Cells/tissues derived from nectin-3 knockout mouse.
• Conjugate: FITC
• Preparation and Storage: Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-PVRL3 antibody

Product Description: The monoclonal antibody 103-A1 recognizes mouse nectin-3. Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calciumindependent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3alpha, -3beta and -3g of which nectin-3alpha is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.

References

1. Satoh-Horikawa, K et al; Nectin-3 a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities. J Biol Chem 2000, 275: 10291 2. Mizoguchi, A et al; Nectin: an adhesion molecule involved in formation of synapses. J Cell Biol, 2002, 156: 555
3. Mueller, S et al; Loss of nectin-2 at Sertoli-spermatid junctions leads to male infertility and correlates with severe spermatozoan head and midpiece malformation, impaired binding to the zona pellucida, and oocyte penetration. Biol Reprod 2003, 69: 1330
4. Guttman, J et al; Evidence that tubulobulbar complexes in the seminiferous epithelium are involved with internalization of adhesion junctions. Biol of Reprod 2004, 71: 548
5. Inagaki, M et al; Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development. Development 2005, 132: 1525
6. Inagaki, M et al; Role of cell adhesion molecule nectin-3 in spermatid development. Genes Cells,2006, 11: 1125
7. Toyama, Y et al; Disruption of ectoplasmic specilizations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies. Asain J Androl 2008, 10: 577

NCBI and Uniprot Product Information

• NCBI GI #: 11386199
• NCBI GeneID: 25945
• NCBI Accession #: NP_056295.1
• NCBI GenBank Nucleotide #: NM_015480.2
• NCBI Official Full Name: nectin-3 isoform 1
• NCBI Official Synonym Full Names: poliovirus receptor-related 3
• NCBI Official Symbol: PVRL3
• NCBI Official Synonym Symbols: PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• NCBI Protein Information: nectin-3; nectin 3; poliovirus receptor-related protein 3
• UniProt Protein Name: Nectin-3
• Protein Family: Nectin
• UniProt Gene Name: PVRL3
• UniProt Synonym Gene Names: PRR3
• UniProt Entry Name: PVRL3_HUMAN

NCBI Description

This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

PVRL3: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: apical junction complex; postsynaptic membrane; cell-cell adherens junction; integral to membrane; plasma membrane

Molecular Function: protein binding; protein homodimerization activity; cell adhesion molecule binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; fertilization; lens morphogenesis in camera-type eye; homophilic cell adhesion; retina morphogenesis in camera-type eye