Rabbit anti-Human PVRL3 Polyclonal Antibody | anti-PVRL3 antibody

PVRL3, CT (PVRL3, PRR3, Poliovirus receptor-related protein 3, CDw113, Nectin-3, CD113) (FITC)

Cat. Num. AAA6339242

• Gene Names: PVRL3; PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PVRL3; Polyclonal Antibody; CT (PVRL3; PRR3; Poliovirus receptor-related protein 3; CDw113; Nectin-3; CD113) (FITC); CD113; anti-PVRL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PVRL3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PVRL3 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 507-536 in the C-terminal region of human PVRL3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MCF-7 cell line lysates (35ug/lane) usingMBS6013249. This demonstrates MBS6013249 detected the PVRL3 protein (arrow).)

Related Product Information for anti-PVRL3 antibody

Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.

Product Categories/Family for anti-PVRL3 antibody

Antibodies; CD Markers

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010 Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Yu, X., et al. Nat. Immunol. 10(1):48-57(2009). Fujito, T., et al. J. Cell Biol. 171(1):165-173(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 11386199
• NCBI GeneID: 25945
• NCBI Accession #: NP_056295.1
• NCBI GenBank Nucleotide #: NM_015480.2
• UniProt Accession #: Q9NQS3; Q6NVZ3; Q8NC05; Q8WVU4; Q9BVA9; Q9Y412; E9PFR0
• NCBI Official Full Name: nectin-3 isoform 1
• NCBI Official Synonym Full Names: poliovirus receptor-related 3
• NCBI Official Symbol: PVRL3
• NCBI Official Synonym Symbols: PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• NCBI Protein Information: nectin-3; nectin 3; poliovirus receptor-related protein 3
• UniProt Protein Name: Poliovirus receptor-related protein 3
• UniProt Gene Name: PVRL3
• UniProt Synonym Gene Names: PRR3
• UniProt Entry Name: PVRL3_HUMAN

NCBI Description

This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

PVRL3: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: apical junction complex; postsynaptic membrane; cell-cell adherens junction; plasma membrane; integral to membrane

Molecular Function: protein binding; protein homodimerization activity; cell adhesion molecule binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; fertilization; lens morphogenesis in camera-type eye; homophilic cell adhesion; retina morphogenesis in camera-type eye