Rat Nectin-2 Monoclonal Antibody
Nectin-2, Mouse, mAb 502-57
Reactivity
Reactivity: Mouse. Cross reactivity: Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
Nectin-2; Monoclonal Antibody; Mouse; mAb 502-57; anti-Nectin-2 antibody
Host
Rat
Reactivity
Reactivity: Mouse. Cross reactivity: Human
Clonality
Monoclonal
Isotype
Rat IgG2a
Form/Format
1 ml (100 ug/ml) purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-Nectin-2 antibody
F, Flow Cytometry (FC/FACS), Immunoassay (IA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Application Notes: The antibody can be used for immuno assays, Western blotting, immuno precipitation, flow cytometry and immunohistology on frozen sections.
Application Use: For flow cytometry, Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Fixation with PFA or acetone/methanol is recommended.
Application Use: For flow cytometry, Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Fixation with PFA or acetone/methanol is recommended.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-Nectin-2 antibody
Product Description: Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice. Monoclonal antibody 502-57 has been raised against the extracellular domain of mouse nectin-2. The antibody cross reacts with human nectin-2.
References
1. Sakisaka, T et al; Different behavior of l-afadin and neurabin-II during the formation and destruction of cell-cell adherens junction. Oncogene 1999, 18: 1609 2. Takahashi, K et al; Nectin/PRR: An immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with afadin, a PDZ domain-containing protein. J Cell Biol 1999, 145: 539
3. Kawabe, H et al; Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells. J Biol Chem 2001, 276: 48350
3. Kawabe, H et al; Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells. J Biol Chem 2001, 276: 48350
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Product Notes
The Nectin-2 (Catalog #AAA584059) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Nectin-2, Mouse, mAb 502-57 reacts with Reactivity: Mouse. Cross reactivity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nectin-2 can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), Immunoassay (IA), Immunoprecipitation (IP), Western Blot (WB). Application Notes: The antibody can be used for immuno assays, Western blotting, immuno precipitation, flow cytometry and immunohistology on frozen sections. Application Use: For flow cytometry, Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Fixation with PFA or acetone/methanol is recommended. Researchers should empirically determine the suitability of the Nectin-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nectin-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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