Western Blot (WB)
(Western Blot analysis of PVRL2 expression in transfected 293T cell line by PVRL2 monoclonal antibody. Lane 1: PVRL2 transfected lysate (Predicted MW: 51.4kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human Nectin 2 Monoclonal Antibody | anti-NECTIN2 antibody
Nectin 2 (Nectin-2, AI325026, AI987993, CD112, Herpes Virus Entry Mediator B, Herpes virus Entry Mediator B, HveB, Murine Herpes Virus Entry Protein B, mHveB, Mph, Poliovirus Receptor Homolog, Poliovirus Receptor-related Protein 2, PRR2, Pvr, Pvrl2, Pvs)
Gene Names
NECTIN2; HVEB; PRR2; CD112; PVRL2; PVRR2
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Nectin 2; Monoclonal Antibody; Nectin 2 (Nectin-2; AI325026; AI987993; CD112; Herpes Virus Entry Mediator B; Herpes virus Entry Mediator B; HveB; Murine Herpes Virus Entry Protein B; mHveB; Mph; Poliovirus Receptor Homolog; Poliovirus Receptor-related Protein 2; PRR2; Pvr; Pvrl2; Pvs); anti-NECTIN2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2A6-2C1
Specificity
Recognizes human PVRL2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-NECTIN2 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to human PVRL2, aa1-480 (AAH03091) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MARAAALLPSRSPPTPLLWPLLLLLFLETGAQDVRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTWQRPDAPANHQNVAAFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVEDEGNYTCEFATFPKGSVRGMTWLRVIAKPKNQAEAQKVTFSQDPTTVALCISKEGRPPARISWLSSLDWEAKETQVSGTLAGTVTVTSRFTLVPSGRADGVTVTCKVEHESFEEPALIPVTLSVRYPPEVSISGYDDNWYLGRTDATLSCDVRSNPEPTGYDWSTTSGTFPTSAVAQGSQLVIHAVDSLFNTTFVCTVTNAVGMGRAEQVIFVRETPRASPRDVGPLVWGAVGGTLLVLLLLAGGSLAFILLRVRRRRKSPGGAGGGASGDGGFYDPKAQVLGNGDPVFWTPVVPGPMEPDGKDEEEEEEEEKAEKGLMLPPPPALEDDMESQLDGSLISRRAVYV
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of PVRL2 expression in transfected 293T cell line by PVRL2 monoclonal antibody. Lane 1: PVRL2 transfected lysate (Predicted MW: 51.4kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of PVRL2 expression in transfected 293T cell line by PVRL2 monoclonal antibody. Lane 1: PVRL2 transfected lysate (Predicted MW: 51.4kD). Lane 2: Non-transfected lysate.)
Immunoprecipitation (IP)
(Immunoprecipitation of PVRL2 transfected lysate using PVRL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PVRL2 rabbit polyclonal antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation of PVRL2 transfected lysate using PVRL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PVRL2 rabbit polyclonal antibody.)
Testing Data
(Detection limit for recombinant GST tagged PVRL2 is 1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged PVRL2 is 1ng/ml as a capture antibody.)
Related Product Information for anti-NECTIN2 antibody
Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin 1, nectin 2 and nectin 3. Nectin 2 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice.
Product Categories/Family for anti-NECTIN2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
51,359 Da
NCBI Official Full Name
Homo sapiens poliovirus receptor-related 2 (herpesvirus entry mediator B), mRNA
NCBI Official Synonym Full Names
nectin cell adhesion molecule 2
NCBI Official Symbol
NECTIN2
NCBI Official Synonym Symbols
HVEB; PRR2; CD112; PVRL2; PVRR2
NCBI Protein Information
nectin-2
Protein Family
NCBI Description
This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Research Articles on NECTIN2
Similar Products
Product Notes
The NECTIN2 (Catalog #AAA6153741) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nectin 2 (Nectin-2, AI325026, AI987993, CD112, Herpes Virus Entry Mediator B, Herpes virus Entry Mediator B, HveB, Murine Herpes Virus Entry Protein B, mHveB, Mph, Poliovirus Receptor Homolog, Poliovirus Receptor-related Protein 2, PRR2, Pvr, Pvrl2, Pvs) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nectin 2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NECTIN2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nectin 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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