Western Blot (WB)
(Western blot detection of NDUFAF1 in K562,Hela cell lysates using NDUFAF1 Rabbit mAb(1:1000 diluted). Predicted band size:38kDa. Observed band size:35kDa.)
Rabbit anti-Human NDUFAF1 Monoclonal Antibody | anti-NDUFAF1 antibody
NDUFAF1 Rabbit mAb
IHC: 1:20-1:100
ICC/IF: 1:50-1:200
FC/FACS: 1:50
IP: 1:20
Western Blot (WB)
(Western blot detection of NDUFAF1 in K562,Hela cell lysates using NDUFAF1 Rabbit mAb(1:1000 diluted). Predicted band size:38kDa. Observed band size:35kDa.)
Western Blot (WB)
(Western blot detection of NDUFAF1 in K562,Hela cell lysates using NDUFAF1 Rabbit mAb(1:1000 diluted). Predicted band size:38kDa. Observed band size:35kDa.)
Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. [provided by RefSeq, Dec 2011]
Uniprot Description
NDUFAF1: Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Belongs to the CIA30 family.
Chromosomal Location of Human Ortholog: 15q11.2-q21.3
Cellular Component: cytoplasm; mitochondrial respiratory chain complex I
Molecular Function: protein binding; unfolded protein binding
Biological Process: mitochondrial electron transport, NADH to ubiquinone; protein complex assembly
Disease: Mitochondrial Complex I Deficiency
Research Articles on NDUFAF1
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Product Notes
The NDUFAF1 ndufaf1 (Catalog #AAA4752898) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFAF1 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFAF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:2000-1:10,000 IHC: 1:20-1:100 ICC/IF: 1:50-1:200 FC/FACS: 1:50 IP: 1:20. Researchers should empirically determine the suitability of the NDUFAF1 ndufaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFAF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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