Mouse NDEL1 Monoclonal Antibody | anti-NDEL1 antibody

NDEL1 antibody

Cat. Num. AAA832285

• Gene Names: NDEL1; EOPA; NDE2; NUDEL; MITAP1; NDE1L1
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: NDEL1 antibody was purified by affinity chromatography.
• Synonyms: NDEL1; Monoclonal Antibody; NDEL1 antibody; Monoclonal NDEL1; Anti-NDEL1; NudE nuclear distribution gene E homolog; NUDEL; MITAP1; Mitosin-associated protein 1; Protein Nudel; EOPA; NDEL 1; NDEL-1; anti-NDEL1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 3B9
• Purity/Purification: NDEL1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 328

• Applicable Applications for anti-NDEL1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:100; FC: 1:100; IHC: 1:150; WB: 1:500-2000
• Biological Significance: NDEL1 a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly tocentrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development.
• Cross-Reactivity: Human
• Immunogen: NDEL1 antibody was raised in mouse using a full length recombinant protein of human NDEL1 (NP_001020750) produced in HEK293 cell as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant NDEL1 protein (red) or empty vector (blue) stained using NDEL1 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NDEL1 protein (Right) or empty vector (Left) detected with NDEL1 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NDEL1 protein in paraffin embedded Human colon tissue using NDEL1 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NDEL1 protein using NDEL1 antibody)

Related Product Information for anti-NDEL1 antibody

Mouse monoclonal NDEL1 antibody

Product Categories/Family for anti-NDEL1 antibody

Differentiation & Development

NCBI and Uniprot Product Information

• NCBI GI #: 71284108
• NCBI GeneID: 81565
• NCBI Accession #: NP_001020750
• NCBI GenBank Nucleotide #: NM_001025579.2
• UniProt Accession #: Q9GZM8; Q86T80; Q8TAR7; Q9UH50; B3KP93; D3DTS0; J3QT32
• Molecular Weight: 37,039 Da
• NCBI Official Full Name: nuclear distribution protein nudE-like 1 isoform A
• NCBI Official Synonym Full Names: nudE neurodevelopment protein 1-like 1
• NCBI Official Symbol: NDEL1
• NCBI Official Synonym Symbols: EOPA; NDE2; NUDEL; MITAP1; NDE1L1
• NCBI Protein Information: nuclear distribution protein nudE-like 1
• UniProt Protein Name: Nuclear distribution protein nudE-like 1
• Protein Family: Nuclear distribution protein
• UniProt Gene Name: NDEL1
• UniProt Synonym Gene Names: EOPA; MITAP1; NUDEL; Protein Nudel
• UniProt Entry Name: NDEL1_HUMAN

NCBI Description

This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Mar 2012]

Uniprot Description

NDEL1: Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Belongs to the nudE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Microtubule-binding

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: axon hillock; kinetochore; kinesin complex; microtubule; centrosome; neurofilament cytoskeleton; leading edge; cytoplasm; nucleolus; nuclear envelope; spindle; cytosol

Molecular Function: protein binding; microtubule binding; beta-tubulin binding; alpha-tubulin binding

Biological Process: cell migration; positive regulation of axon regeneration; central nervous system neuron axonogenesis; positive regulation of axon extension; establishment of chromosome localization; neuron migration; nuclear envelope disassembly; microtubule nucleation; proteolysis; retrograde axon cargo transport; inner cell mass cell proliferation; chromosome segregation; mitotic centrosome separation; neurofilament cytoskeleton organization and biogenesis; establishment of mitotic spindle orientation; centrosome localization; vesicle transport along microtubule; cerebral cortex radially oriented cell migration; mitotic cell cycle