Mouse NCK1 Monoclonal Antibody | anti-NCK1 antibody

NCK1 Antibody

Cat. Num. AAA9602441

• Gene Names: NCK1; NCK; nck-1; NCKalpha
• Reactivity: Human, Monkey
• Applications: Western Blot, Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
• Purity: Affinity-Chromatography
• Synonyms: NCK1; Monoclonal Antibody; NCK1 Antibody; Cytoplasmic protein NCK1; Melanoma Nck protein; MGC12668; NCK 1; NCK adaptor protein 1; NCK alpha; NCK; NCK tyrosine kinase; Nck-1; NCK1_HUMAN; NCKalpha; Non catalytic region of tyrosine kinase; SH2/SH3 adaptor protein NCK alpha; SH2/SH3 adaptor protein NCK-alpha; anti-NCK1 antibody

• Host: Mouse
• Reactivity: Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: NCK1 antibody detects endogenous levels of total NCK1
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 313

• Applicable Applications for anti-NCK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: ELISA: 1:10000; WB: 1:500-1:2000; IHC: 1:200-1:1000; FC/FACS: 1:200-1:400
• Immunogen: Purified recombinant fragment of human NCK1 expressed in E. Coli
• Subcellular Location: Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Figure 1: Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein. (Expected MW is 44.9 kDa))

Related Product Information for anti-NCK1 antibody

Description: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.
Subunit Structure: Interacts (via SH2 domain and SH3 domain 2) with EGFR. Interacts with PAK1 and SOS1. Interacts (via SH3 domains) with PKN2. Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7. This interaction is required for nuclear import. Part of a complex containing PPP1R15B, PP1 and NCK1. Interacts with RALGPS1. Interacts with CAV2 (tyrosine phosphorylated form). Interacts with ADAM15. Interacts with FASLG. Directly interacts with RASA1. Interacts with isoform 4 of MINK1. Interacts with FLT1 (tyrosine phosphorylated). Interacts with KDR (tyrosine phosphorylated). Interacts (via SH2 domain) with EPHB1; activates the JUN cascade to regulate cell adhesion. Interacts with EPHA2. Interacts (via SH2 domain) with PDGFRB (tyrosine phosphorylated). Interacts with the inactive form of EIF2AK2/PKR.
Post-translational Modifications: Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.
Similarity: Only the first and third SH3 domains seem to be involved in RASA1-binding; the second SH3 domain and the SH2 domains do not seeem to be involved.

NCBI and Uniprot Product Information

• NCBI GI #: 299829184
• NCBI GeneID: 4690
• NCBI Accession #: NP_001177725.1
• NCBI GenBank Nucleotide #: NM_001190796.2
• UniProt Accession #: P16333; B7Z751; D3DNE3
• Molecular Weight: Observed: 47 kDa; Predicted: 43 kDa
• NCBI Official Full Name: cytoplasmic protein NCK1 isoform 2
• NCBI Official Synonym Full Names: NCK adaptor protein 1
• NCBI Official Symbol: NCK1
• NCBI Official Synonym Symbols: NCK; nck-1; NCKalpha
• NCBI Protein Information: cytoplasmic protein NCK1
• UniProt Protein Name: Cytoplasmic protein NCK1
• Protein Family: Cytoplasmic protein
• UniProt Gene Name: NCK1
• UniProt Synonym Gene Names: NCK; Nck-1

NCBI Description

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]

Uniprot Description

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.