Mouse Natural Cytotoxicity Receptor NKp46 Monoclonal Antibody | anti-NKp46 antibody
Mouse Anti Human Natural Cytotoxicity Receptor NKp46
Applications
Western Blot
Purity
NKp46 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Synonyms
Natural Cytotoxicity Receptor NKp46; Monoclonal Antibody; Mouse Anti Human Natural Cytotoxicity Receptor NKp46; NKp46 Antibody; Mouse Anti Human; Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; hNKp46; NK-p46; NKp46; NK cell-activating receptor; Lymphocyte antigen 94 homolog; CD335 antigen; NCR1; LY94; NCRNKp46; CD335; anti-NKp46 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 heavy chain and kappa light chain
Clone Number
Pn1D9AT
Purity/Purification
NKp46 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sterile Filtered colorless solution.
Sterile Filtered colorless solution.
Applicable Applications for anti-NKp46 antibody
Western Blot (WB)
Application Notes
NKp46 antibody has been tested by immunofluorescent staining with flow cytometric analysis and by Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Immunogen
Anti-human NKp46 mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NKp46 amino acids 22-255 purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
12 months at -20 degree C. 1 month at 4 degree C.
Related Product Information for anti-NKp46 antibody
Introduction: A natural cytotoxicity receptor (NCR) NKp46 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. The three known NCRs include NKp46 and NKp30, which are expressed on circulating NKcells, and NKp44, which is expressed only on activating NK cells. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells, pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium. The Lysis of tumor cells by NK-cells involves recognition by NKp46 of heparan sulfate moieties of membrane heparan sulfate proteoglycans. Furthermore, NKp46 is a surface receptor involved in NK-cell cell death by apoptosis. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. The extracellular Ig-like domain of NKp46 (22-255aa) is purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. In addition, engagement of the antigen with the monoclonal antibody stimulates intracellular calcium levels and the synthesis of cytokines. CD59 is an NKp46 coreceptor (by physical association) together they activate cytotoxicity of human NK-cells, their engagement results in tyrosine phosphorylation of CD3-zeta chains associated with NKp46. Reduced cell surface expression of NKp46 and other NK-cell receptors is linked to the impaired NK-cell cytolytic function in viremic HIV-1 infection.
Product Categories/Family for anti-NKp46 antibody
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Product Notes
The NKp46 (Catalog #AAA140187) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Natural Cytotoxicity Receptor NKp46 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). NKp46 antibody has been tested by immunofluorescent staining with flow cytometric analysis and by Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the NKp46 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Natural Cytotoxicity Receptor NKp46, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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