Rabbit anti-Human N Cadherin Monoclonal Antibody | anti-CDH2 antibody

Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal

Cat. Num. AAA8104664

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: N Cadherin; Monoclonal Antibody; Recombinant Anti-N Cadherin Antibody; Rabbit Monoclonal; N-Cadherin/CD325/CDH2 Antibody; Rabbit MAb; cadherin 2; type 1; N-cadherin (neuronal); Anti-CD325 Antibody; Anti-CDHN Antibody; Anti-CDw325 Antibody; Anti-NCAD Antibody; anti-CDH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 020
• Specificity: Human N-Cadherin/CD325/CDH2
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CDH2 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; ICC/IF: 1:20-1:100; FC/FACS/FCM: 1:25-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human N-Cadherin/CD325/CDH2 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human N-Cadherin/CD325/CDH2 (rh N-Cadherin/CD325/CDH2).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of Human CDH2 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CDH2 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to membrane.)

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of N-Cadherin on HeLa cells. HeLa cells were harvested with (Left) or without (Right) trypsinization [please note, the epitope is sensitive to trypsin] and stained with either purified Rabbit IgG isotype control (dashed line) or Rabbit Anti-Human CD325 antibody [solid line, and Clone R001 (upper), Clone R020 (bottom)] at matching concentrations, then a FITC-conjugated second step antibody.)

Western Blot (WB)

(Anti-CDH2 rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:99 kDaObserved band size:130 kDa)

Related Product Information for anti-CDH2 antibody

Cadherins are calcium-dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass transmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression.

Product Categories/Family for anti-CDH2 antibody

Early Mesodermal Lineage Markers; Canonical Wnt Pathway

References

Jones M, et al. (2002) N-cadherin upregulation and function in response of smooth muscle cells to arterial injury. Arterioscler Thromb Vasc Biol. 22(12): 1972-7.Nagi C, et al. (2005) N-cadherin expression in breast cancer: correlation with an aggressive histologic variant--invasive micropapillary carcinoma. Breast Cancer Res Treat. 94(3): 225-35.Schrick C, et al. (2007) N-cadherin regulates cytoskeletally associated IQGAP1/ERK signaling and memory formation. Neuron. 55(5): 786-98.Li K, et al. (2010) Downregulation of N-cadherin expression inhibits invasiveness, arrests cell cycle and induces cell apoptosis in esophageal squamous cell carcinoma. Cancer Invest. 28(5): 479-86.

NCBI and Uniprot Product Information

• NCBI GI #: 14589889
• NCBI GeneID: 1000
• NCBI Accession #: NP_001783.2
• NCBI GenBank Nucleotide #: NM_001792.3
• UniProt Accession #: P19022; Q14923; Q8N173; B0YIY6
• Molecular Weight: 99,809 Da
• NCBI Official Full Name: cadherin-2 preproprotein
• NCBI Official Synonym Full Names: cadherin 2, type 1, N-cadherin (neuronal)
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
• UniProt Protein Name: Cadherin-2
• Protein Family: Cadherin
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin
• UniProt Entry Name: CADH2_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH2: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Interacts with CDCP1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: cell-cell adherens junction; focal adhesion; lamellipodium; apical plasma membrane; integral to membrane; plasma membrane; synapse; intercellular junction; fascia adherens; catenin complex

Molecular Function: protein binding; gamma-catenin binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; alpha-catenin binding

Biological Process: heterophilic cell adhesion; intercellular junction assembly and maintenance; muscle cell differentiation; cell migration; glial cell differentiation; positive regulation of MAPKKK cascade; calcium-dependent cell-cell adhesion; blood vessel morphogenesis; positive regulation of muscle cell differentiation; striated muscle cell differentiation; cell adhesion; homophilic cell adhesion