Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with Myogenin MAb (F5D))
Mouse Myogenin Monoclonal Antibody | anti-MYOG antibody
Myogenin (bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4, Myogenin myogenin factor 4) (HRP)
Gene Names
MYOG; MYF4; myf-4; bHLHc3
Reactivity
Feline, Human, Mouse, Porcine, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
Myogenin; Monoclonal Antibody; Myogenin (bHLHc3; cb553; Class C basic helix-loop-helix protein 3; Myf-4; MYF4; MYOG; Myogenic factor 4; Myogenin myogenin factor 4) (HRP); bHLHc3; Myogenin myogenin factor 4; anti-MYOG antibody
Host
Mouse
Reactivity
Feline, Human, Mouse, Porcine, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
F5D
Specificity
Recognizes human Myogenin. Species Crossreactivity: mouse, rat, feline, and porcine. Others not known.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-MYOG antibody
Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes
WB: 0.5-1.0ug/ml
IHC-F/P: 0.5-1.0ug/ml for 30 minutes at RT
IP: 0.5-1ug/500ug protein lysate
Applications are based on unconjugated antibody.
IHC-F/P: 0.5-1.0ug/ml for 30 minutes at RT
IP: 0.5-1ug/500ug protein lysate
Applications are based on unconjugated antibody.
Immunogen
Human myogenin recombinant protein. Cellular Localization: Nuclear
Conjugate
HRP
Positive Control
Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with Myogenin MAb (F5D))
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with Myogenin MAb (F5D))
Product Categories/Family for anti-MYOG antibody
References
1. Wang NP et. al. Am J Pathol 1995, 147:1799-1810.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25kDa
NCBI Official Full Name
myogenin
NCBI Official Synonym Full Names
myogenin (myogenic factor 4)
NCBI Official Symbol
MYOG
NCBI Official Synonym Symbols
MYF4; myf-4; bHLHc3
NCBI Protein Information
myogenin; myogenic factor 4; class C basic helix-loop-helix protein 3
UniProt Protein Name
Myogenin
Protein Family
UniProt Gene Name
MYOG
UniProt Synonym Gene Names
BHLHC3; MYF4; bHLHc3; Myf-4
UniProt Entry Name
MYOG_HUMAN
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Product Notes
The MYOG myog (Catalog #AAA6123536) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Myogenin (bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4, Myogenin myogenin factor 4) (HRP) reacts with Feline, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Myogenin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB). WB: 0.5-1.0ug/ml IHC-F/P: 0.5-1.0ug/ml for 30 minutes at RT IP: 0.5-1ug/500ug protein lysate Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MYOG myog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myogenin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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