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Western Blot (WB) (Western Blot analysis of reduced lysates from C2C12 cells using Myogenin antibody (5 ug/ml) (left). Bands were visualized using of Mouse IgG HRP antibody. Immunocytochemistry of fixed and permeabilized C2C12 cell line using Myogenin antibody (1 ug/ml) antibody followed by Mouse IgG FITC antibody (right).)

Mouse anti-Human Myogenin Monoclonal Antibody | anti-MYOG antibody

Mouse monoclonal Myogenin antibody

Gene Names
MYOG; MYF4; myf-4; bHLHc3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Functional Assay
Purity
Purified by affinity chromatography.
Synonyms
Myogenin; Monoclonal Antibody; Mouse monoclonal Myogenin antibody; Myogenin antibody; anti-MYOG antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
F5D
Specificity
Human
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
224
Applicable Applications for anti-MYOG antibody
Flow Cytometry (FC/FACS), Functional Assay
Application Notes
FC: <= 1 ug/test
Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10? to 10? cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.

Western Blot (WB)

(Western Blot analysis of reduced lysates from C2C12 cells using Myogenin antibody (5 ug/ml) (left). Bands were visualized using of Mouse IgG HRP antibody. Immunocytochemistry of fixed and permeabilized C2C12 cell line using Myogenin antibody (1 ug/ml) antibody followed by Mouse IgG FITC antibody (right).)

Western Blot (WB) (Western Blot analysis of reduced lysates from C2C12 cells using Myogenin antibody (5 ug/ml) (left). Bands were visualized using of Mouse IgG HRP antibody. Immunocytochemistry of fixed and permeabilized C2C12 cell line using Myogenin antibody (1 ug/ml) antibody followed by Mouse IgG FITC antibody (right).)
Product Categories/Family for anti-MYOG antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,037 Da
NCBI Official Full Name
myogenin
NCBI Official Synonym Full Names
myogenin
NCBI Official Symbol
MYOG
NCBI Official Synonym Symbols
MYF4; myf-4; bHLHc3
NCBI Protein Information
myogenin
UniProt Protein Name
Myogenin
Protein Family
UniProt Gene Name
MYOG
UniProt Synonym Gene Names
BHLHC3; MYF4; bHLHc3; Myf-4

NCBI Description

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]

Uniprot Description

myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin DNA binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity

Biological Process: mRNA transcription from RNA polymerase II promoter; muscle cell fate commitment; negative regulation of cell proliferation; positive regulation of muscle atrophy; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; regulation of satellite cell proliferation; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle development; striated muscle atrophy

Research Articles on MYOG

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Product Notes

The MYOG myog (Catalog #AAA5306825) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal Myogenin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myogenin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Assay. FC: <= 1 ug/test. Researchers should empirically determine the suitability of the MYOG myog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myogenin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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