Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 MAb (MYD712))
Mouse MyoD1 Monoclonal Antibody | anti-MyoD1 antibody
MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (PE)
IHC-F/P: 0.5-1ug/ml for 30 minutes at RT
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
WB: 0.25-0.5ug/ml
Applications are based on unconjugated antibody.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 MAb (MYD712))
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 MAb (MYD712))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
Protein type: DNA-binding; RNA splicing; Transcription factor
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding
Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on MyoD1
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Product Notes
The MyoD1 myod1 (Catalog #AAA6123526) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (PE) reacts with Chicken, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MyoD1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB). FC/FACS: 0.5-1ug/million cells IHC-F/P: 0.5-1ug/ml for 30 minutes at RT IP: 0.5-1ug/500ug protein lysate IF: 0.5-1ug/ml WB: 0.25-0.5ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MyoD1 myod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MyoD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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