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Western Blot (WB)
(Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody. MBS9200092 was diluted at 1:2000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.)
Mouse Myc Tag Monoclonal Antibody
Myc Tag Antibody
Applications
Western Blot, ELISA
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
Myc Tag; Monoclonal Antibody; Myc Tag Antibody; Tag from c-Myc protein; anti-Myc Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
9E10
Specificity
KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Applicable Applications for anti-Myc Tag antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2,000
Crown Antibody
Yes
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody. MBS9200092 was diluted at 1:2000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody. MBS9200092 was diluted at 1:2000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of lysate from 12Tag protein, using Myc Tag Antibody. MBS9200092 was diluted at 1:4000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from 12Tag protein, using Myc Tag Antibody. MBS9200092 was diluted at 1:4000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Myc-tagged recombinant protein was expressed in COS7 cells. 20 ug of cell lysate was loaded onto a gel to test the Myc tag antibody (MBS9200092).)
Western Blot (WB)
(Myc-tagged recombinant protein was expressed in COS7 cells. 20 ug of cell lysate was loaded onto a gel to test the Myc tag antibody (MBS9200092).)
Western Blot (WB)
(Myc western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Myc antibody detected the Myc protein (arrow).)
Western Blot (WB)
(Myc western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Myc antibody detected the Myc protein (arrow).)
Western Blot (WB)
(Myc western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Myc antibody detected the Myc protein (arrow).)
Western Blot (WB)
(Myc western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Myc antibody detected the Myc protein (arrow).)
Related Product Information for anti-Myc Tag antibody
Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. MyBioSource's anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.
Product Categories/Family for anti-Myc Tag antibody
References
Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.
Similar Products
Product Notes
The Myc Tag (Catalog #AAA9200092) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Myc Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2,000. Researchers should empirically determine the suitability of the Myc Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myc Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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