Mouse anti-Human MX1 Monoclonal Antibody | anti-MX1 antibody

MX1 (MX1, Interferon-induced GTP-binding protein Mx1, Interferon-induced protein p78, Interferon-regulated resistance GTP-binding protein MxA, Myxoma resistance protein 1, Myxovirus resistance protein 1, Interferon-induced GTP-binding protein Mx1, N-termi

Cat. Num. AAA6121722

• Gene Names: MX1; MX; MxA; IFI78; IFI-78K
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: MX1; Monoclonal Antibody; MX1 (MX1; Interferon-induced GTP-binding protein Mx1; Interferon-induced protein p78; Interferon-regulated resistance GTP-binding protein MxA; Myxoma resistance protein 1; Myxovirus resistance protein 1; N-termi; N-terminally processed; anti-MX1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 474CT4.1.5
• Specificity: Recognizes human MX1.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MX1 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 617-646 of human MX1, UniProt Accession #P20591. Species Sequence Homology: porcine
• Conjugate: PE
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in T47D cell line lysates using (35ug/lane). This demonstrates that detected the MX1 protein (arrow).)

Related Product Information for anti-MX1 antibody

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. Two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-MX1 antibody

Antibodies; Interferon

References

1. Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010). 2. van der Voort, L.F., et al. Neurology 75(14):1228-1233(2010). 3. Ching, J.C., et al. J. Infect. Dis. 201(12):1899-1908(2010). 4. Zhijian, Y., et al. Virol. J. 7, 278 (2010). 5. Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010)

NCBI and Uniprot Product Information

• NCBI GI #: 222136619
• NCBI GeneID: 4599
• NCBI Accession #: NP_001138397.1
• NCBI GenBank Nucleotide #: NM_001144925.2
• UniProt Accession #: P20591; Q86YP5; Q96CI3; B2RDA5; B3KU10; C9IYV7; C9J8D6; C9JN19; C9JN88; C9JUL1; C9JZS6; D3DSI8
• Molecular Weight: 55,661 Da
• NCBI Official Full Name: interferon-induced GTP-binding protein Mx1 isoform a
• NCBI Official Synonym Full Names: MX dynamin like GTPase 1
• NCBI Official Symbol: MX1
• NCBI Official Synonym Symbols: MX; MxA; IFI78; IFI-78K
• NCBI Protein Information: interferon-induced GTP-binding protein Mx1
• UniProt Protein Name: Interferon-induced GTP-binding protein Mx1
• Protein Family: Interferon-induced GTP-binding protein
• UniProt Gene Name: MX1
• UniProt Synonym Gene Names: IFI-78K
• UniProt Entry Name: MX1_HUMAN

Uniprot Description

MX1: Dynamin-like GTPase acting as a cell-autonomous host restriction factor against many viral pathogens. Shows activity against influenza viruses, rhabdovirus VSV, bunyavirus LACV, Thogoto virus, measles virus, Hantaan virus, Coxsackie virus CVB, Rift Valley fever virus RVFV, hepatitis B virus HBV, and Crimean- Congo hemorrhagic fever virus CCHFV. Belongs to the dynamin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Apoptosis

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: negative regulation of viral genome replication; apoptosis; metabolic process; cytokine and chemokine mediated signaling pathway; response to virus; innate immune response; defense response; signal transduction; defense response to virus