Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (SPM200).)
Mouse anti-Human MUC3 (Mucin 3) Monoclonal Antibody | anti-MUC3A antibody
MUC3 (Mucin 3) Mouse Monoclonal Antibody
Gene Names
MUC3A; MUC3; MUC-3A
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
MUC3 (Mucin 3); Monoclonal Antibody; MUC3 (Mucin 3) Mouse Monoclonal Antibody; Intestinal mucin-3A; intestinal mucin-like; MUC3; MUC3A; MUC3B; mucin 3; intestinal; mucin 3A; cell surface associated; mucin 3B; anti-MUC3A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
SPM200
Specificity
It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
3323
Applicable Applications for anti-MUC3A antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Cytoplasmic and Cell Surface
Immunogen
A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
Hu-Chromosome Location
7q22.1
Positive Control
LS174T cells or colon carcinoma and normal colon tissue.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (SPM200).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (SPM200).)
References
Apostolopoulos V, et. al. Journal of Gastroenterology and Hepatology, 1995, 10:555-61.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
1100kDa
NCBI Official Full Name
mucin-3A
NCBI Official Synonym Full Names
mucin 3A, cell surface associated
NCBI Official Symbol
MUC3A
NCBI Official Synonym Symbols
MUC3; MUC-3A
NCBI Protein Information
mucin-3A
UniProt Protein Name
Mucin-3A
Protein Family
UniProt Gene Name
MUC3A
UniProt Synonym Gene Names
MUC-3ACurated
UniProt Entry Name
MUC3A_HUMAN
NCBI Description
The mucin genes encode epithelial glycoproteins, some of which are secreted and some membrane bound. Each of the genes contains at least one large domain of tandemly repeated sequence that encodes the peptide sequence rich in serine and/or threonine residues, which carries most of the O-linked glycosylation (Gendler and Spicer, 1995 [PubMed 7778880]).[supplied by OMIM, Aug 2008]
Uniprot Description
MUC3A: Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. May be involved in ligand binding and intracellular signaling. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q22
Cellular Component: Golgi lumen
Biological Process: O-glycan processing
Disease: Inflammatory Bowel Disease 11
Research Articles on MUC3A
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Product Notes
The MUC3A muc3a (Catalog #AAA438676) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MUC3 (Mucin 3) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MUC3 (Mucin 3) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the MUC3A muc3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC3 (Mucin 3), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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