Flow Cytometry (FC/FACS)
(Figure-1: Intra cellular Flow analysis of mTIM-3 in Raw cells using 1 ug/10^6 cells of mTIM-3 antibody (Clone: ABM3F21). Green represents isotype control; red represents anti-mTIM-3 antibody. Goat anti-Rat FITC conjugate was used as secondary antibody.)
Rat anti-Mouse mTIM-3 Monoclonal Antibody | anti-mTIM-3 antibody
Monoclonal Antibody to mTIM-3 (Clone: ABM3F21)
Protein G Chromatography
Protein G Chromatography
For long-term storage, store at -20 degree C.
Avoid repeat freezing and thawing
Flow Cytometry (FC/FACS)
(Figure-1: Intra cellular Flow analysis of mTIM-3 in Raw cells using 1 ug/10^6 cells of mTIM-3 antibody (Clone: ABM3F21). Green represents isotype control; red represents anti-mTIM-3 antibody. Goat anti-Rat FITC conjugate was used as secondary antibody.)
Flow Cytometry (FC/FACS)
(Figure-1: Intra cellular Flow analysis of mTIM-3 in Raw cells using 1 ug/10^6 cells of mTIM-3 antibody (Clone: ABM3F21). Green represents isotype control; red represents anti-mTIM-3 antibody. Goat anti-Rat FITC conjugate was used as secondary antibody.)
NCBI and Uniprot Product Information
Uniprot Description
Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:18006747). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556006, PubMed:18006747). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits (). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (PubMed:21807895). Expressed on Treg cells can inhibit Th17 cell responses (). Receptor for LGALS9. Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6 (PubMed:22863785). Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (PubMed:20937702). However, the function as receptor for LGALS9 has been challenged (). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (PubMed:24567532). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent (PubMed:20083673). May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells (PubMed:19224762). Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells (PubMed:20083673). Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha (PubMed:18006747). In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (PubMed:22842346). Can enhance mast cell production of Th2 cytokines Il-4, IL-6 and IL-13 (PubMed:17620455). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9. In contrast, shown to suppress NK cell-mediated cytotoxicity (). Negatively regulates NK cell function in LPS-induced endotoxic shock (PubMed:25337993).
Research Articles on mTIM-3
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Product Notes
The mTIM-3 havcr2 (Catalog #AAA668983) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to mTIM-3 (Clone: ABM3F21) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's mTIM-3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: 2-4 ug/10^6 cells. Researchers should empirically determine the suitability of the mTIM-3 havcr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mTIM-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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