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Western Blot (WB) (Figure-1: Western blot analysis of MSI-1. Anti-MSI1 was used at 0.5 ug/ml on Recombinant and 2 ug/ml in Pancrease and HepG2 Lysates.)

Mouse anti-Human MSi-1 Monoclonal Antibody | anti-MSi-1 antibody

Monoclonal Antibody to MSi-1 (Clone: ABM5D50)

Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Purified
Protein G Chromatography
Synonyms
MSi-1; Monoclonal Antibody; Monoclonal Antibody to MSi-1 (Clone: ABM5D50); RNA-binding protein Musashi homolog 1; Musashi-1; anti-MSi-1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
ABM5D50
Purity/Purification
Purified
Protein G Chromatography
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
362
Applicable Applications for anti-MSi-1 antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Facs anaysis: 2-4 ug/10^6 Cells
WB analysis: 2-4 ug/ml
Antigen
MSI1
Immunogen Information
A partial length recombinant protein of Msi-1 (amino acid 19-263) was used as the immunogen for this antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Figure-1: Western blot analysis of MSI-1. Anti-MSI1 was used at 0.5 ug/ml on Recombinant and 2 ug/ml in Pancrease and HepG2 Lysates.)

Western Blot (WB) (Figure-1: Western blot analysis of MSI-1. Anti-MSI1 was used at 0.5 ug/ml on Recombinant and 2 ug/ml in Pancrease and HepG2 Lysates.)

Flow Cytometry (FC/FACS)

(Figure-2: Intra cellular flow analysis of Msi-1 on HePG2 cells using 2 ug/10^6 cells of Msi-1 antibody (Clone: ABM5D50). Green represents isotype control; red represents anti-Msi-1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody)

Flow Cytometry (FC/FACS) (Figure-2: Intra cellular flow analysis of Msi-1 on HePG2 cells using 2 ug/10^6 cells of Msi-1 antibody (Clone: ABM5D50). Green represents isotype control; red represents anti-Msi-1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody)

Flow Cytometry (FC/FACS)

(Figure-3: Intra cellular flow analysis of Msi-1 on HeLa cells using 2 ug/10^6 cells of Msi-1 antibody (Clone: ABM5D50). Green represents isotype control; red represents anti-Msi-1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

Flow Cytometry (FC/FACS) (Figure-3: Intra cellular flow analysis of Msi-1 on HeLa cells using 2 ug/10^6 cells of Msi-1 antibody (Clone: ABM5D50). Green represents isotype control; red represents anti-Msi-1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
Product Categories/Family for anti-MSi-1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,125 Da
NCBI Official Full Name
RNA-binding protein Musashi homolog 1
NCBI Official Synonym Full Names
musashi RNA binding protein 1
NCBI Official Symbol
MSI1
NCBI Protein Information
RNA-binding protein Musashi homolog 1
UniProt Protein Name
RNA-binding protein Musashi homolog 1
UniProt Gene Name
MSI1
UniProt Synonym Gene Names
Musashi-1

NCBI Description

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq, Jul 2008]

Uniprot Description

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system ().

Research Articles on MSi-1

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Product Notes

The MSi-1 msi1 (Catalog #AAA669035) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to MSi-1 (Clone: ABM5D50) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MSi-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). Facs anaysis: 2-4 ug/10^6 Cells WB analysis: 2-4 ug/ml. Researchers should empirically determine the suitability of the MSi-1 msi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MSi-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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