Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRRF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human MRRF Monoclonal Antibody | anti-MRRF antibody
MRRF antibody
Gene Names
MRRF; RRF; MRFF; MTRRF
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-A affinity chromatography
Synonyms
MRRF; Monoclonal Antibody; MRRF antibody; Ribosome-recycling factor mitochondrial isoform 1; Ribosome-recycling factor; mitochondrial isoform 1; MRFF; MTRRF; RRF; anti-MRRF antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT7D10
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
262
Applicable Applications for anti-MRRF antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Recommended starting dilution is 1:1000.
Antigen Species
Human
Immunogen
Recombinant human MRRF (56-262aa) purified from E. coli
Clone Note
Anti-human MRRF mAb, clone AT7D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MRRF protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRRF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRRF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-MRRF antibody
Mitochondrial ribosome recycling factor, also known as MRRF, is a 262 amino acid protein that belongs to the ribosome recycling factor (RRF) family. Localized to mitochondria, MRRF is responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. It may increase the efficiency of translation by recycling ribosomes from one round of translation to another. MRRF belongs to the RRF family. 8 isoforms of the human protein are produced by alternative splicing.
Product Categories/Family for anti-MRRF antibody
References
Checcarelli, N. et al. (May 1994). J Neurol Sci. 123 (1-2):74-9; Janosi, L. et al. (1996). Biochimie. 78 (11-12):959-69.; Zavialov, A.V. et al. (Jun 2005). Mol Cell. 10;18 (6):675-86.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,511 Da
NCBI Official Full Name
ribosome-recycling factor, mitochondrial isoform 1
NCBI Official Synonym Full Names
mitochondrial ribosome recycling factor
NCBI Official Symbol
MRRF
NCBI Official Synonym Symbols
RRF; MRFF; MTRRF
NCBI Protein Information
ribosome-recycling factor, mitochondrial
UniProt Protein Name
Ribosome-recycling factor, mitochondrial
UniProt Gene Name
MRRF
UniProt Synonym Gene Names
RRF
NCBI Description
This gene encodes a ribosome recycling factor, which is a component of the mitochondrial translational machinery. The encoded protein, along with mitochondrial elongation factor 2, functions in ribosomal recycling at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA. A pseudogene of this gene has been identified on chromosome X. [provided by RefSeq, Oct 2016]
Uniprot Description
Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another ().
Research Articles on MRRF
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Product Notes
The MRRF mrrf (Catalog #AAA200227) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MRRF antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MRRF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the MRRF mrrf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRRF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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