Mouse anti-Human MPP1 Monoclonal Antibody | anti-MPP1 antibody

MPP1 (55kD Erythrocyte Membrane Protein, p55, Membrane Protein, Palmitoylated 1, DXS552E, EMP55) (PE)

Cat. Num. AAA6158878

• Gene Names: MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MPP1; Monoclonal Antibody; MPP1 (55kD Erythrocyte Membrane Protein; p55; Membrane Protein; Palmitoylated 1; DXS552E; EMP55) (PE); anti-MPP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1C3-1D11
• Specificity: Recognizes human MPP1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MPP1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-466 from human MPP1 (AAH02392) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (77kD).)

Western Blot (WB)

(MPP1 monoclonal antibody Western Blot analysis of MPP1 expression in K-562.)

Western Blot (WB)

(Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody. Lane 1: MPP1 transfected lysate (52.3kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged MPP1 is ~3ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of MPP1 over-expressed 293 cell line, cotransfected with MPP1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-MPP1 antibody

Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. [provided by RefSeq]

Product Categories/Family for anti-MPP1 antibody

Antibodies; Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 38197472
• NCBI GeneID: 4354
• Molecular Weight: 49,854 Da
• NCBI Official Full Name: Homo sapiens membrane protein, palmitoylated 1, 55kDa, mRNA
• NCBI Official Synonym Full Names: membrane palmitoylated protein 1
• NCBI Official Symbol: MPP1
• NCBI Official Synonym Symbols: MRG1; PEMP; AAG12; EMP55; DXS552E
• NCBI Protein Information: 55 kDa erythrocyte membrane protein
• Protein Family: Mitochondrial-processing peptidase

NCBI Description

This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]