Mouse Anti-Human IgG (gamma chain specific) Secondary Antibody
Mouse Anti-Human IgG (Fc)-AP
Reactivity
Human
Applications
ELISA, Immunoblot, Immunocytochemistry, Immunohistochemistry, Western Blot
Synonyms
Mouse Anti-Human IgG (gamma chain specific); Monoclonal Secondary Antibody; Mouse Anti-Human IgG (Fc)-AP; Mouse Anti-Human IgG (Fc)- Alkaline Phosphatase-Labeled; Mouse Anti-Human IgG (gamma chain specific) secondary antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
JDC-10
Specificity
Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA, FLISA, and/pr flow cytometry. May also react with IgG from other species.
Form/Format
Alkaline Phosphatase (AP) Conjugate
Applicable Applications for Mouse Anti-Human IgG (gamma chain specific) secondary antibody
Research Application(s): Indirect immunofluorescent staining; ELISA; FLISA; Immunoblotting; Immunohistochemistry (Frozen Section) (IHC-F); Immunocytochemistry (ICC)
Application Notes
WORKING DILUTIONS:
Flow Cytometry:
Purified (UNLB) antibody: < or = 1ug/10^6 cells
BIOT conjugate: < or = 1ug/10^6 cells
PE conjugate: < or = 0.2 ug/10^6 cells
ELISA:
AP conjugate: 1:1,000-1:4,000
HRP conjugate: 1:2,000-1:8,000
BIOT conjugate: 1:5,000-1:10,000
Other applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
Flow Cytometry:
Purified (UNLB) antibody: < or = 1ug/10^6 cells
BIOT conjugate: < or = 1ug/10^6 cells
PE conjugate: < or = 0.2 ug/10^6 cells
ELISA:
AP conjugate: 1:1,000-1:4,000
HRP conjugate: 1:2,000-1:8,000
BIOT conjugate: 1:5,000-1:10,000
Other applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA for conformance to characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin eyes, or cloting. Wear eye of face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Each reagent is stable for the period shown on the bottle label if stored as directed.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Each reagent is stable for the period shown on the bottle label if stored as directed.
Product Categories/Family for Mouse Anti-Human IgG (gamma chain specific) secondary antibody
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Product Notes
The Mouse Anti-Human IgG (gamma chain specific) (Catalog #AAA674011) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IgG (Fc)-AP reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mouse Anti-Human IgG (gamma chain specific) can be used in a range of immunoassay formats including, but not limited to, Research Application(s): Indirect immunofluorescent staining; ELISA; FLISA; Immunoblotting; Immunohistochemistry (Frozen Section) (IHC-F); Immunocytochemistry (ICC). WORKING DILUTIONS: Flow Cytometry: Purified (UNLB) antibody: < or = 1ug/10^6 cells BIOT conjugate: < or = 1ug/10^6 cells PE conjugate: < or = 0.2 ug/10^6 cells ELISA: AP conjugate: 1:1,000-1:4,000 HRP conjugate: 1:2,000-1:8,000 BIOT conjugate: 1:5,000-1:10,000 Other applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the Mouse Anti-Human IgG (gamma chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse Anti-Human IgG (gamma chain specific), Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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