Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant MOBKL2B protein (red) or empty vector (blue) stained using MOBKL2B antibody)
Mouse MOBKL2B Monoclonal Antibody | anti-MOBKL2B antibody
MOBKL2B antibody
Gene Names
MOB3B; MOB1D; C9orf35; MOBKL2B
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
MOBKL2B antibody was purified by affinity chromatography.
Synonyms
MOBKL2B; Monoclonal Antibody; MOBKL2B antibody; Monoclonal MOBKL2B; Anti-MOBKL2B; MOB1 Mps One Binder kinase activator-like 2B; MOB3B; FLJ13204; Mob1 homolog 2b; FLJ23916; Protein Mob3B; MOBKLB 2; MOBKLB-2; anti-MOBKL2B antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5C7
Purity/Purification
MOBKL2B antibody was purified by affinity chromatography.
Form/Format
Affinity purified from mouse ascites, supplied as liquid in PBS, pH 7.3, with 1% BSA, 50% Glycerol, and 0.02% NAN3.
Concentration
0.62mg/ml (varies by lot)
Sequence Length
216
Applicable Applications for anti-MOBKL2B antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:2000
IF: 1:100
WB: 1:2000
Biological Significance
MOBKL2B shares similarity with the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. This gene is located on the opposite strand as the interferon kappa precursor (IFNK) gene.
Cross-Reactivity
Human
Immunogen
Full length human recombinant protein of human MOBKL2B produced in HEK293T cell.
Preparation and Storage
4 degree C shipping. Upon receipt store at -20 degree C. Avoid repeat freeze-thaws.
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant MOBKL2B protein (red) or empty vector (blue) stained using MOBKL2B antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant MOBKL2B protein (red) or empty vector (blue) stained using MOBKL2B antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MOBKL2B protein (Right) or empty vector (Left) detected with MOBKL2B antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MOBKL2B protein (Right) or empty vector (Left) detected with MOBKL2B antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant MOBKL2B protein using MOBKL2B antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant MOBKL2B protein using MOBKL2B antibody)
Related Product Information for anti-MOBKL2B antibody
Mouse monoclonal MOBKL2B antibody
Product Categories/Family for anti-MOBKL2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,464 Da
NCBI Official Full Name
MOB kinase activator 3B
NCBI Official Synonym Full Names
MOB kinase activator 3B
NCBI Official Symbol
MOB3B
NCBI Official Synonym Symbols
MOB1D; C9orf35; MOBKL2B
NCBI Protein Information
MOB kinase activator 3B
UniProt Protein Name
MOB kinase activator 3B
UniProt Gene Name
MOB3B
UniProt Synonym Gene Names
MOBKL2B
UniProt Entry Name
MOB3B_HUMAN
NCBI Description
The protein encoded by this gene shares similarity with the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. This gene is located on the opposite strand as the interferon kappa precursor (IFNK) gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MOBKL2B: May regulate the activity of kinases. Belongs to the MOB1/phocein family.
Chromosomal Location of Human Ortholog: 9p21.2
Molecular Function: protein binding; metal ion binding
Research Articles on MOBKL2B
Similar Products
Product Notes
The MOBKL2B mob3b (Catalog #AAA832214) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MOBKL2B can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the MOBKL2B mob3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MOBKL2B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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