Mouse anti-Human MOAP1 Monoclonal Antibody | anti-MOAP1 antibody

MOAP1 (Modulator of Apoptosis 1, MAP-1, MAP1, Paraneoplastic Antigen Ma4, PNMA4)

Cat. Num. AAA646369

• Gene Names: MOAP1; MAP-1; PNMA4
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MOAP1; Monoclonal Antibody; MOAP1 (Modulator of Apoptosis 1; MAP-1; MAP1; Paraneoplastic Antigen Ma4; PNMA4); Anti -MOAP1 (Modulator of Apoptosis 1; anti-MOAP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4A11
• Specificity: Recognizes human MOAP1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MTLRLLEDWCRGMDMNPRKALLIAGISQSCSVAEIEEALQAGLAPLGEYRLLGRMFRRDENRKVALVGLTAETSHALVPKEIPGKGGIWRVIFKPPDPDNTFLSRLNEFLAGEGMTVGELSRALGHENGSLDPEQGMIPEMWAPMLAQALEALQPALQCLKYKKLRVFSGRESPEPGEEEFGRWMFHTTQMIKAWQVPDVEKRRRLLESLRGPALDVIRVLKINNPLITVDECLQALEEVFGVTDNPRELQVKYLTTYQKDEEKLSAYVLRLEPLLQKLVQRGAIERDAVNQARLDQVIAGAVHKTIRRELNLPEDGPAPGFLQLLVLIKDYEAAEEEEALLQAILEGNFT

• Applicable Applications for anti-MOAP1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Full length recombinant corresponding to aa1-352 from human MOAP1 (AAH15044) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (64.72kD).)

Testing Data

(Detection limit for recombinant GST tagged MOAP1 is ~1ng/ml as a capture antibody.)

Related Product Information for anti-MOAP1 antibody

A novel Bax-associating protein, named MAP-1 (Modulator of Apoptosis), mediates caspase-dependent apoptosis in mammalian cells when overexpressed. MAP-1 homodimerizes and associates with the proapoptotic Bax and the prosurvival Bcl-2 and Bcl-X(L) of the Bcl-2 family in vitro and in vivo in mammalian cells. These data suggest that MAP-1 mediates apoptosis through a mechanism that involves binding to Bax.

Product Categories/Family for anti-MOAP1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 19923584
• NCBI GeneID: 64112
• NCBI Accession #: NP_071434.2
• NCBI GenBank Nucleotide #: NM_022151.4
• UniProt Accession #: Q96BY2; Q9H833; Q9HAS1; B2RDF6
• Molecular Weight: 39,513 Da
• NCBI Official Full Name: modulator of apoptosis 1
• NCBI Official Synonym Full Names: modulator of apoptosis 1
• NCBI Official Symbol: MOAP1
• NCBI Official Synonym Symbols: MAP-1; PNMA4
• NCBI Protein Information: modulator of apoptosis 1; MAP1; paraneoplastic antigen Ma4; paraneoplastic antigen like 4; paraneoplastic Ma antigen family member 4
• UniProt Protein Name: Modulator of apoptosis 1
• Protein Family: Modulator of apoptosis
• UniProt Gene Name: MOAP1
• UniProt Synonym Gene Names: PNMA4; MAP-1; MAP1
• UniProt Entry Name: MOAP1_HUMAN

NCBI Description

The protein encoded by this gene was identified by its interaction with apoptosis regulator BAX protein. This protein contains a Bcl-2 homology 3 (BH3)-like motif, which is required for the association with BAX. When overexpressed, this gene has been shown to mediate caspase-dependent apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Required for death receptor-dependent apoptosis. When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Ref.5

Subunit structure: Homodimer. Under normal circumstances, held in an inactive conformation by an intramolecular interaction. Binding to RASSF1 isoform A (RASSF1A) relieves this inhibitory interaction and allows further binding to BAX. Binds also to BCL2 and BCLX. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Interacts with TRIM39. Interacts with RASSF6

By similarity. Ref.5 Ref.6

Tissue specificity: Ubiquitously expressed with higher levels in heart and brain.

Domain: The BH3-like domain is required for association with BAX and for mediating apoptosis. The three BH domains (BH1, BH2, and BH3) of BAX are all required for mediating protein-protein interaction.

Post-translational modification: Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1, this modification is inhibited by TRIM39. Ref.7

Sequence similarities: Belongs to the PNMA family.

Sequence caution: The sequence BAB14788.1 differs from that shown. Reason: Frameshift at position 102.