Western Blot (WB)
(Western blot analysis of MKP-3 expression in non-transfected (A) and human MKP-3 transfected (B) 293T whole cell lysates.)
Mouse MKP-3 Monoclonal Antibody | anti-MKP-3 antibody
MKP-3 Antibody
IHC: 1:50-1:500
IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate)
Western Blot (WB)
(Western blot analysis of MKP-3 expression in non-transfected (A) and human MKP-3 transfected (B) 293T whole cell lysates.)
Western Blot (WB)
(Western blot analysis of MKP-3 expression in non-transfected (A) and human MKP-3 transfected (B) 293T whole cell lysates.)
Testing Data
(Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic staining of respiratory epithelial cells.)
Testing Data
(Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic staining of respiratory epithelial cells.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MKP-3: a non-receptor, dual-specificity phosphoprotein phosphatase. Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This protein inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two alternatively spliced isoforms have been described.
Protein type: Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48; EC 3.1.3.16
Chromosomal Location of Human Ortholog: 12q22-q23
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity
Biological Process: response to drug; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of fibroblast growth factor receptor signaling pathway; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to nitrosative stress; dorsal/ventral pattern formation; toll-like receptor signaling pathway; regulation of endodermal cell fate specification; innate immune response; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway; inactivation of MAPK activity
Disease: Hypogonadotropic Hypogonadism 19 With Or Without Anosmia
Research Articles on MKP-3
Similar Products
Product Notes
The MKP-3 dusp6 (Catalog #AAA9435177) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MKP-3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MKP-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. WB: 1:100-1:1000 IHC: 1:50-1:500 IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate). Researchers should empirically determine the suitability of the MKP-3 dusp6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MKP-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
