Mouse Mitosin Monoclonal Antibody
Mitosin Monoclonal Antibody
Applications
Immunoblot, Western Blot, Immunohistochemistry, Immunoprecipitation
Synonyms
Mitosin; Monoclonal Antibody; Mitosin Monoclonal Antibody; Anti-Mitosin; anti-Mitosin antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a kappa
Specificity
Anti-Mitosin [Murine MAb recognizes mitosin, a 350 kDa phosphoprotein involved in mitotic-phase progression.]
This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
Form/Format
100 ug Protein G purified antibody in PBS, pH 7.4
Applicable Applications for anti-Mitosin antibody
Immunoblot, Immunohistochemistry, Immunoprecipitation
Application Notes
Immunoblotting: use at 1-10 ug/ml.
Immunoprecipitation: use at 1-10 ug/ml.
Immunohistochemistry: use at 10 ug/mlwith frozen or paraffin-embeddedsections.
Positive controls: Human tissue containingrapidly proliferating cells, such aslymphoid germinal centers.
Immunoprecipitation: use at 1-10 ug/ml.
Immunohistochemistry: use at 10 ug/mlwith frozen or paraffin-embeddedsections.
Positive controls: Human tissue containingrapidly proliferating cells, such aslymphoid germinal centers.
Antigen
GST fusion protein expressed inE. coli corresponding to aa 1759-2093 of human mitosin.
Stabilizer
None
Preservatives
None. Available on request.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
Product Categories/Family for anti-Mitosin antibody
References
Allred, DC et al. (1997) Lab Invest 15A(69); Zhu, X et al. (1995), Mol Cell Biol 15:5017-5029.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
mitosin
NCBI Description
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]
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Product Notes
The Mitosin (Catalog #AAA190324) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mitosin can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunohistochemistry, Immunoprecipitation. Immunoblotting: use at 1-10 ug/ml. Immunoprecipitation: use at 1-10 ug/ml. Immunohistochemistry: use at 10 ug/mlwith frozen or paraffin-embeddedsections. Positive controls: Human tissue containingrapidly proliferating cells, such aslymphoid germinal centers. Researchers should empirically determine the suitability of the Mitosin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitosin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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