Mouse Mitofusin-2 Monoclonal Antibody | anti-Mfn2 antibody

Mitofusin-2 Monoclonal Antibody

Cat. Num. AAA191341

• Gene Names: Mfn2; Fzo; D630023P19Rik
• Applications: Immunoblot, Western Blot, Immunofluorescence
• Synonyms: Mitofusin-2; Monoclonal Antibody; Mitofusin-2 Monoclonal Antibody; anti-Mfn2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: This antibody recognizes human, mouse, and rat Mitofusin-2. It does not crossreact with Mitofusin-1.
• Form/Format: 100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
• Sequence Length: 757

• Applicable Applications for anti-Mfn2 antibody: Immunoblot, Immunofluorescence (IF)
• Application Notes: Immunoblot: 1-5ug/ml. A band of ~90kDa is detected.; IF: 10ug/ml
• Immunogen: Fusion protein corresponding to aa 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. This sequence is 97% identical to rat, 92% identical to human, and ~55% identical to Mitofusin-1.

Testing Data

(Detection of Mitofusin-2 in rat brain lysate at 5ug/ml. )

Testing Data

(Detection of Mitofusin-2 in neuroblastoma cell line SK-N-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) Mitofusin-2 stain.)

Related Product Information for anti-Mfn2 antibody

Mitofusin-2 (Mfn2) is a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2 and hereditary motor and sensory neuropathy VI, both of which are disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 120407048
• NCBI GeneID: 170731
• NCBI Accession #: NP_573464.2
• NCBI GenBank Nucleotide #: NM_133201.3
• UniProt Accession #: Q80U63; Q3V3B8; Q80WP4; Q80XK3; Q8BHF0; Q8BKV5; Q8R535; Q923X2; A2A7Y7; A8Y5E4
• Molecular Weight: 68,938 Da
• NCBI Official Full Name: mitofusin-2
• NCBI Official Synonym Full Names: mitofusin 2
• NCBI Official Symbol: Mfn2
• NCBI Official Synonym Symbols: Fzo; D630023P19Rik
• NCBI Protein Information: mitofusin-2
• UniProt Protein Name: Mitofusin-2
• Protein Family: Mitofusin
• UniProt Gene Name: Mfn2
• UniProt Synonym Gene Names: Kiaa0214; Marf; HSG protein

Uniprot Description

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:23620051). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation (). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (PubMed:23921556). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (PubMed:23921556).