Mouse anti-Rat Mincle Monoclonal Antibody
Mincle, Rat, mAb WEN43
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay
Synonyms
Mincle; Monoclonal Antibody; Rat; mAb WEN43; Marcophage inducible C-type lectin; CLR; CLECSF9; CLEC4E; anti-Mincle antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
WEN43
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-Mincle antibody
Flow Cytometry (FC/FACS), Functional Studies (FS)
Application Notes
Flow Cytometry: Antibody WEN43 stains the extracellular domain of Mincle transfected 293 cells. WEN43 does not bind to other receptors in the rat APLEC family.
The typical starting working dilution is 1:50.
The typical starting working dilution is 1:50.
Immunogen
Rat mincle-Fcgamma2b fusion protein
Positive Control
BW5417 or 293T cells
Preparation and Storage
Store at 4 degree C. Under recommended storage conditions, product is stable for at least one year.
Related Product Information for anti-Mincle antibody
The monoclonal antibody WEN43 recognizes rat macrophage inducible C-type lectin (Mincle). This receptor is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. For instance, Mincle is involved in mediating inflammatory responses to necrotic cells and is a pathogen sensor for Mycobacterium tuberculosis. It is a member of the Dendritic Cell Inhibitory Receptor (DCIR) group of C-type lectin receptors (CLRs), which are expressed mainly by antigen presenting cells and are encoded in a cluster in the natural killer gene complex. CLRs play crucial roles in defense against pathogens and recognize a variety of carbohydrate moieties. Ligand binding to CLRs leads to the activation of NF-kB via a Syk/CARD9 signaling module. Mincle was originally identified as a lipopolysaccharide-inducible protein in macrophages and has been shown to stimulate inflammatory responses to fungi, although the ligands and signaling pathways involved have not been defined. WEN43 does not bind to other receptors in the rat APLEC family.
References
1.Lobato-Pascual, A et al; Mincle, the receptor for mycobacterial cord factor, forms a functional receptor complex with MCL and FcepsilonRI-gamma. Eur J Immunol 2013, 43: 3167
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Product Notes
The Mincle (Catalog #AAA584776) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mincle, Rat, mAb WEN43 reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mincle can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Studies (FS). Flow Cytometry: Antibody WEN43 stains the extracellular domain of Mincle transfected 293 cells. WEN43 does not bind to other receptors in the rat APLEC family. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the Mincle for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mincle, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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