Rabbit anti-Human, Mouse Mib1 Monoclonal Antibody | anti-Mib1 antibody

Mib1 Antibody

Cat. Num. AAA9436650

• Gene Names: MIB1; MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: Mib1; Monoclonal Antibody; Mib1 Antibody; DAPK-interacting protein 1 antibody; Dip 1 antibody; DIP-1 antibody; Dip1 antibody; E3 ubiquitin protein ligase MIB 1 antibody; E3 ubiquitin protein ligase MIB1 antibody; E3 ubiquitin-protein ligase mib1 antibody; KIAA1323 antibody; LVNC7 antibody; MIB antibody; mib1 antibody; MIB1_HUMAN antibody; Mind bomb homolog 1 antibody; Mindbomb E3 ubiquitin protein ligase 1 antibody; Ubiquitin ligase mind bomb antibody; Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody; ZZANK2 antibody; ZZZ6 antibody; anti-Mib1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: JG69-34
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-Mib1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200; ICC: 1:50-1:200
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein within N-terminal human Mib1.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Mib1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Mib1 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Mib1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Mib1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Mib1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-Mib1 antibody

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

Product Categories/Family for anti-Mib1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 30348954
• NCBI GeneID: 57534
• NCBI Accession #: NP_065825
• NCBI GenBank Nucleotide #: NM_020774.3
• UniProt Accession #: Q86YT6; Q2TB37; Q68D01; Q6YI51; Q8NBY0; Q8TCB5; Q8TCL7; Q9P2M3; B0YJ38
• Molecular Weight: Predicted: 89; 102; 111 kDa; ; Observed: 103 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase MIB1
• NCBI Official Synonym Full Names: mindbomb E3 ubiquitin protein ligase 1
• NCBI Official Symbol: MIB1
• NCBI Official Synonym Symbols: MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
• NCBI Protein Information: E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; ubiquitin ligase mind bomb; zinc finger ZZ type with ankyrin repeat domain protein 2
• UniProt Protein Name: E3 ubiquitin-protein ligase MIB1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MIB1
• UniProt Synonym Gene Names: DIP1; KIAA1323; ZZANK2; DIP-1
• UniProt Entry Name: MIB1_HUMAN

NCBI Description

This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013]

Uniprot Description

MIB1: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation. Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: centrosome; postsynaptic density; cytoplasm; plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: blood vessel development; somitogenesis; negative regulation of neuron differentiation; Notch signaling pathway; in utero embryonic development; protein ubiquitination; positive regulation of endocytosis; endocytosis; heart looping; neural tube formation

Disease: Left Ventricular Noncompaction 7