Rat anti-Canine MHC Class II Monomorphic Monoclonal Antibody

MHC Class II Monomorphic (MaxLight 650)

Cat. Num. AAA6256190

• Reactivity: Canine
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by protein G affinity chromatography from tissue culture supernatant.
• Synonyms: MHC Class II Monomorphic; Monoclonal Antibody; MHC Class II Monomorphic (MaxLight 650); anti-MHC Class II Monomorphic antibody

• Host: Rat
• Reactivity: Canine
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: YKIX334.2
• Specificity: Recognizes canine MHC Class II Monomorphic. Species Crossreactivity: Hooded Seal
• Purity/Purification: Purified by protein G affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight650.

• Applicable Applications for anti-MHC Class II Monomorphic antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Con A activated canine peripheral blood cells.
• Conjugate: MaxLight650
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Canine MHC Class II: FITC.)

Product Categories/Family for anti-MHC Class II Monomorphic antibody

Antibodies; MHC

References

1. Cobbold, S. and Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the first International Canine Leucocyte Antigen Workshop. Tissue Antigens 43: 137-154. 2. Watson, C.J.E. et al. (1994) Immunosuppression of canine renal allograft recipients by CD4 and CD8 monoclonal antibodies. Tissue Antigens 43: 155-162. 3. Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi. Clin Exp Immunol.146: 303-11. 4. Arau'jo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis. Vet Immunol Immunopathol. 141: 64-75. 5. Bonnefont-Rebeix, C. et al. (2007) Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC). Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):134-9. 6. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies. Cell Immunol. 263: 88-98. 7. Mito, K. et al. (2010) IFNgamma markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models. Cancer Res. 70: 7093-101. 8. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs. Immunology. 105: 499-508. 9. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi. Am J Trop Med Hyg. 70: 618-24. 10. Schu:tze, N. et al. (2009) Inactivated parapoxvirus ovis activates canine blood phagocytes and T lymphocytes. Vet Microbiol. 137: 260-7. 11. Liu, Y. et al. (2000) Immunosuppressant-free allotransplantation of the tracheaThe antigenicity of tracheal grafts can be reduced by removing the epithelium and mixed glands from the graft by detergent treatment. J Thorac Cardiovasc Surg. 120: 108-14. 12. Larsen, A.K. et al. (2013) Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro. PLoS One. 8: e70186.