Mouse anti-Human MGMT Monoclonal Antibody | anti-Mgmt antibody

MGMT (O6-Methylguanine DNA Methyltransferase)

Cat. Num. AAA604112

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A chromatography.
• Synonyms: MGMT; Monoclonal Antibody; MGMT (O6-Methylguanine DNA Methyltransferase); Anti -MGMT (O6-Methylguanine DNA Methyltransferase); anti-Mgmt antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 6A129
• Specificity: Recognizes the human MGMT protein. Reactivity is confirmed with human T cell leukemia CEM/CCRF cells.
• Purity/Purification: Affinity Purified; Purified by Protein A chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Mgmt antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Immunohistochemistry, Western Blot and Flow Cytometry.; Dilution: Western Blot: 1-3ug/ml; Flow Cytometry: 5-10ug/ml; Immunohistochemistry: 10ug/ml; Note: Staining results for immunohistochemical assays with formalin-fixed, paraffin-embedded tissue sections may be greatly enhanced with an antigen retrieval pre-treatment step using citrate buffer, pH 6.0.
• Immunogen: Recombinant human MGMT protein purified from E. coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Mgmt antibody

O6-methylguanine-DNA transferase (MGMT) is a ubiquitous 22kD DNA repair protein, involved in the repair of mutagenic, carcinogenic, and cytotoxic O6-alkylguanine adducts, including primary alkyl adducts, induced by the chloroethylating and methylating agents used in the treatment of cancers. MGMT transfers alkyl groups from the O6 position of guanine in DNA to a cysteine residue in its sequence, restoring the integrity of DNA. This transfer is irreversible and inactivates MGMT; a cell's ability to withstand damage is directly related to the number

Product Categories/Family for anti-Mgmt antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 127071
• NCBI GeneID: 25332
• UniProt Accession #: P24528
• Molecular Weight: 22,244 Da
• NCBI Official Full Name: Methylated-DNA--protein-cysteine methyltransferase
• NCBI Official Synonym Full Names: O-6-methylguanine-DNA methyltransferase
• NCBI Official Symbol: Mgmt
• NCBI Protein Information: methylated-DNA--protein-cysteine methyltransferase; O6-alkylguanine-DNA alkyltransferase; O6-methylguanine-DNA methyltranferase; O-6-methylguanine-DNA-alkyltransferase; 0-6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
• UniProt Protein Name: Methylated-DNA--protein-cysteine methyltransferase
• Protein Family: Probable methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: Mgmt
• UniProt Synonym Gene Names: MGMT
• UniProt Entry Name: MGMT_RAT

NCBI Description

enzyme involved in DNA repair of O(6)-alkylguanine which is the major mutagenic and carcinogenic lesion in DNA [RGD, Feb 2006]

Uniprot Description

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: EC 2.1.1.63; Apoptosis; Nuclear receptor co-regulator; Methyltransferase; DNA repair, damage

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding

Biological Process: response to drug; methylation; response to ethanol; DNA dealkylation; positive regulation of DNA repair; response to folic acid; response to toxin; DNA repair; regulation of caspase activity; response to organic cyclic substance; response to DNA damage stimulus