Mouse anti-Human MGMT Monoclonal Antibody | anti-MGMT antibody

MGMT (O6-Methylguanine DNA Methyltransferase) (FITC)

Cat. Num. AAA6243342

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MGMT; Monoclonal Antibody; MGMT (O6-Methylguanine DNA Methyltransferase) (FITC); anti-MGMT antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q434
• Specificity: Recognizes MGMT in human tissues, tumors, cells or xenograft lines at ~22-25kD. Gives weak cross-reactivity with MGMT from mouse, hampster and rat cells.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-MGMT antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Recombinant protein corresponding to human MGMT (O6-methylguanine-DNA methyltransferase) expressed in E. coli. Swiss/Uniprot Accession: P16455
• Conjugate: FITC
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western Blot Analysis: Representative lot data. HEK293 lysate (Lane 1), HeLa lysate (Lane 2), HepG2 lysate (Lane 3), Jurkat lysate (Lane 4) and PC3 lysate (Lane 5) were resolved by electrophoresis, transferred to PVDF membrane and probed withMBS601697. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein MGMT (~22kD).)

Western Blot (WB)

(Western Blot: Representative lot data. Jurkat lysate resolved by electrophoresis, transferred to PVDF membrane & probed with MBS601697 Proteins were visualized using goat anti-mouse secondary Ab conjugated to HRP chemiluminescence detection system. Arrow indicates MGMT (22kD). )

Immunohistochemistry (IHC)

(Immunohistochemistry staining of MGMT in human breast cancer usingMBS601697.)

Immunofluorescence (IF)

(Confolcal fluorescent analysis of HeLa using MBS601697 (Red). Actin filaments have been labeled with AlexaFluor® 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining.)

Product Categories/Family for anti-MGMT antibody

Antibodies; Enzymes; Methyltransferase

References

1. Brent, T.P., et al. Cancer Res. 50:58-61(1990). 2. VonWronski, M.A., and Brent, T.P. Carcinogenesis 15:577 (1994). 3. Allay, J.A., et al. 1995. Blood. 85: 3342. 4. Harris, L.C., et al. 1996. Cancer Res. 56: 2029. 5. Harris, L.C., et al. 1996. Carcinogenesis. 17: 219. 6. Zaidi, N.H., et al. 1996. Clin. Cancer Res. 2: 577. 7. Allay, J.A., et al. 1996. Clin. Cancer Res. 2: 1353-1359. 8. Reese, J.S., et al. 1996. PNAS USA 93: 14088. 9. Kokkinakis, D.M., et al. 1997. Br. J. Cancer 75: 779-788. 10. Chen, Z.P., et al. 1997. Mol. Pharmacol (in press). 11. Gerson, S.L. et al. 1996. Blood. 88: 1649-1655 12. Rossi, D., et al. (2004). Haematologica. 89: 154-164. 13. Brock, MV., et al. (2003). Haematologia. 9: 2912-2919.

NCBI and Uniprot Product Information

• NCBI GI #: 127069
• NCBI GeneID: 4255
• NCBI Accession #: P16455.1
• NCBI GenBank Nucleotide #: NM_002412.2
• UniProt Accession #: P16455; Q5VY78
• Molecular Weight: 21,646 Da
• NCBI Official Full Name: Methylated-DNA--protein-cysteine methyltransferase
• NCBI Official Synonym Full Names: O-6-methylguanine-DNA methyltransferase
• NCBI Official Symbol: MGMT
• NCBI Protein Information: methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
• UniProt Protein Name: Methylated-DNA--protein-cysteine methyltransferase
• Protein Family: Probable methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: MGMT
• UniProt Synonym Gene Names: MGMT
• UniProt Entry Name: MGMT_HUMAN

Uniprot Description

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator; DNA repair, damage; Apoptosis

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; DNA binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity

Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation